摘要
目的探讨环磷酰胺对腹腔巨噬细胞酸性磷酸酶的影响。方法将20只小鼠随机分为实验组和对照组,每组10只。实验组小鼠背部皮下注射环磷酰胺50mg·kg-1,对照组小鼠背部皮下注射生理盐水0.5mL,2组均为每日1次,连续5d。在实验组和对照组注射的第3天,同时用淀粉肉汤免疫小鼠,每日1次,在第3次免疫后的3～4h,取腹腔液,在37℃酸性磷酸酶作用液中,温育30min;经甲醛固定和硫化胺作用后,蒸馏水漂洗,晾干,显微镜下检查。结果经环磷酰胺处理后,实验组小鼠腹腔巨噬细胞酸性磷酸酶活性明显降低。巨噬细胞平均密度图象分析表明,对照组中平均密度为127.36±9.52,实验组中的平均密度为65.37±6.37,2组间有极显著差异(P<0.01)。结论环磷酰胺对小鼠的非特异性免疫系统功能有抑制作用。
Objective To study the influence on the acid phosphatase of peritoneal macrophage with cyclophosphamide. Methods Twenty mice were divided into experimental group and control group randomly,ten mice in each group. Cyclophosphamide（ 50 mg·kg^-1） and physiological saline（ 0.5 mL）was injected into the back with subcutaneous injections in the experimental group and in the control group respectively. The two groups all were one time per day for 5 days continuously. The mice in two groups were immunized with starch broth in the third day of the injection with one time per day. After the third immunization for 3 -4 h,the peritoneal fluid were taken out and incubated for 30 min in acid phosphatase action liquid at 37 ℃. Then they were fixed with formalin and acted with ammonium sulfide, rinsed with distilled water, open-air drying then they were examined with microscope. Results After hypodermic with cyclophosphamide,the activity of acid phosphatase in peritoneal macrophage in experimental group was decline obviously, Average density image analysis of macrophage indicated that the average density of control group was 127.36 ± 9.52 and experiment group was 65.37 ± 6.37. And there was significant deviation between the two groups（ P 〈 0.01 ）. Conclusion Cyctophosphamide has the inhibitation to the nonspecific immunity system of mouse.
出处
《新乡医学院学报》
CAS
2008年第4期366-368,共3页
Journal of Xinxiang Medical University
