摘要
目的:探讨小檗碱对高果糖饲养诱导胰岛素抵抗大鼠肝细胞核因子(hepatocyte nuclearfactor-4α,HNF-4α)表达的影响以及其改善胰岛素抵抗的分子机制.方法:高果糖饲料喂养SD大鼠6wk,建立胰岛素抵抗模型;大鼠分为4组:正常对照组(普通饮食)、普通饮食+小檗碱处理组、高果糖饮食组及高果糖饮食+小檗碱组;小檗碱按187.5mg/(kg·d)灌服4wk;测定血糖、血清胰岛素、胰岛素抵抗指数及甘油三酯的变化;用RT-PCR法及免疫印迹法观察肝脏HNF-4α基因及蛋白的表达.结果:高果糖饮食组胰岛素、胰岛素抵抗指数HOMA及甘油三酯水平较正常对照组均明显升高(均P<0.01),而高果糖饮食+小檗碱组与高果糖饮食组比较上述指标明显下降(51.62±5.68vs64.91±7.87,P<0.01;12.40±1.76vs16.06±3.32,P<0.01;11.16±1.58vs14.46±2.99,P<0.05),小檗碱可促使肝脏表达下降的HNF-4α恢复.结论:小檗碱可改善胰岛素抵抗,其机制可能与促进HNF-4α的表达有关.
AIM： To investigate the effects of berberine on the expression of hepatocyte nuclear factor4α （HNF- 4α） in rats with fructose-induced insulin resistance.
METHODS： SD rats were given high-fructose diet for 6 wk, All rats were divided into 4 groups： control group, common diet ＋ berberine group, high-fructose diet group and high-fructose diet ＋ berberine group. Berberine was given （187.5 mg/kg per day）. After the experimental period of 10 wk, Plasma glucose, insulin and tri- glyceride levels were measured. HOMA insulin resistance index （HOMA-IR） was assayed. Fur-thermore, RT-PCR and Western Blot were used to detect and analyze the expression of HNF-4α and protein in liver.
RESULTS： Compared with control diet, fructose feeding induced hyperinsulinemia, higher HOMA-IR and raised triglyceride （all P 〈 0.01）. Compared with fructose-fed rats, indicators above decreased significantly in the high-fructose diet ＋ berberine group （51.62 ± 5.68 vs 64.91 ± 7.87, P 〈 0.01; 12.40 ± 1.76 vs 16.06 ± 3.32, P 〈 0.01; 11.16 ± 1.58 vs 14.46 ± 2.99, P 〈 0.05）. The mRNA and protein expression of HNF-4α was decreased in the fructose-fed rats, while berberine promoted its expression.
CONCLUSION： Berberine improves fructose- induced insulin resistance probably by increasing HNF-4α expression.
出处
《世界华人消化杂志》
CAS
北大核心
2008年第15期1681-1684,共4页
World Chinese Journal of Digestology
基金
国家自然科学基金资助项目
No.30500685~~
关键词
胰岛素抵抗
小檗碱
肝脏
肝细胞核因子
4α
高果糖
Insulin resistance
Berberine
Liver
Hepatocyte nuclear factor-4α
High fructose
