摘要
建立了液相色谱-氢化物发生-原子荧光联用同时检测预混料中阿散酸和洛克沙胂的实验方法。饲料样品采用20％甲醇水溶液为提取液，用超声、振荡提取方法，用symmetry shield TM RP18柱150mm×4．6mm，5μm分离，以含0．1％甲酸的0．05mol／L磷酸二氢钾溶液：甲醇（95：5）为流动相，流速为1．0mL／min，进样量100μm条件下，于193．7nm波长处检测。实验条件下：阿散酸、洛克沙胂的浓度在0．2—4μg／mL范围内，含量与峰面积呈良好的线性关系，R^2分别为0．9992，0．9990。三个添加水平上方法回收率为96．40％-107．42％，RSD 0．86％-5．20％。
Development of simultaneous analysis of p-arsenilic and roxarsone in animal feed hy liquid chromatography-hydrodide-atomic-flurescence spectrometry,adopting 20% （ratio of volume） methylalcolhol aqueous solution as extracts to pretreatment samples, ultrasound bath, shaked, then through C18 chromatography pillar separates, using 0.05 mol/L K2HPO4：CH3OH（95：5 ）as mobile phase, at a rate of 1 mL/min,inject 100μL,detected at 193.7 nm,the result showed that：the Linear range was 0.2 -4μg/mL, with linear relative coefficient,p-arsenilic acid R^2 is 0.999 2, roxarsone R^2 is 0. 999 0, the average recoveries of three different addition level were 96.40% - 107.42%, RSD was 0.86% - 5.20%.
出处
《应用化工》
CAS
CSCD
2008年第6期694-696,710,共4页
Applied Chemical Industry
关键词
阿散酸
洛克沙胂
饲料
液相色谱-氢化物发生-原子荧光
同时检测
p-arsenilic acid
roxarsone
feed
Liquid chromatography-hydrodide-atomic-flurescence spec- trometry
simultaneous analysis
