摘要
采用蛋白质组双向电泳技术,对室温及-20℃贮存6个月的茶花蜂花粉蛋白质组差异进行了比较。结果表明,室温贮存6个月的茶花蜂花粉总蛋白质为261个,-20℃贮存6个月的茶花蜂花粉总蛋白质为396个,室温贮存6个月的茶花蜂花粉蛋白质较-20℃贮存6个月的茶花蜂花粉蛋白质数目明显减少,说明室温贮存可能会引起茶花蜂花粉内蛋白质的损失。同时发现,两种不同贮存条件下的茶花蜂花粉,也存在着蛋白质丰度的差异,其中24个蛋白质在室温贮存时丰度较大,而在低温贮存条件下丰度较小;112个蛋白质在室温贮存下丰度较小,而在低温贮存下丰度较大。可见,不同温度条件下贮存蜂花粉会对蛋白质种类及丰度均产生较大影响。冷冻贮存蜂花粉是一种比较好的贮存方法。
To better understand the molecular mechanism of honeybee pollen at the protein level during storage, two-dimensional gel electrophoresis technology was used to investigate honeybee pollen of Camellia sinensis stored at room temperature and at - 20 ℃ for 6 months respectively. Result showed that 261 protein spots are separated from honeybee pollen stored at room temperature, while 396 protein spots are detected from honeybee pollen stored at -20 ℃. The reducing number of total proteins in honeybee pollen stored at room temperature is less than that at -20℃. Moreover, results indicated that storage of honeybee pollen at room temperature might causes protein degradation. Simultaneously, the abundance of certain protein is different at different storage temperatures. 24 proteins abundances are higher in honeybee pollen stored at room temperature than that at - 20℃. Furthermore, 112 protein spots show lower abundance in honeybee pollen stored at room temperature than that at - 20℃. Results indicated that protein and protein abundance might be affected by storage temperature, honeybee pollen storing at - 20℃ is probably a better storage method to maintain its quality.
出处
《食品科学》
CAS
CSCD
北大核心
2008年第6期438-443,共6页
Food Science
基金
“十一五”国家科技支撑计划重点项目(2006BAD06B04
2006BAD12B08)
中国农业科学院杰出人才课题启动基金项目
关键词
茶花蜂花粉
贮存温度
蛋白质组
双向凝胶电泳
honeybee pollen of Camellia sinensis
two-dimensional gel electrophoresis
proteome
storage temperature
