摘要
通过复制犬急性坏死性胰腺炎（ＡＮＰ）模型，探讨ＡＮＰ时肠通透性变化及其与肠道细菌．移位的关系。杂种犬１５只，分ＡＮＰ组（ｎ＝８）和对照组（ｎ＝７）。气相色谱法测定尿中乳果糖和甘露醇的含量；每日血培养；第７天活杀后各组脏器标本作细菌培养。结果发现，ＡＮＰ组尿中乳果糖／甘露醇比率高出对照组２～１２倍，发病后第２天最为显著（Ｐ＜０．０１），ＡＮＰ犬都出现了肠道细菌移位，血培养阳性率为８／８，以发病后４８小时内最高，镜检证实ＡＮＰ组肠粘膜上皮绒毛脱落。对照组只有２只犬在肠系膜淋巴结中培养出细菌，其它脏器和血培养全部阴性。本研究提示：ＡＮＰ后４８小时肠通透性即出现病理性升高，肠粘膜屏障遭到严重破坏，发生肠道细菌移位。
The changes of intestinal permeability and bacterial translocation were studied in dog model of acute necrotizing pancreatitis(ANP).Fifteen dogs were randomly divided into two groups.In ANP group(n=8),ANP was induced,and 7 dogs served as control.All dogs were fed with lactulose and mannitol on day 1,2,4and 7,respectively.Gas chromatography was apllied to determine the levels of lactulose and mannitol in the urine within 6h. Blood samples were cultured every day.All animals were sacrificed on day 7.Tissues of organs were colleted for histological and microbiological studies.The results showed that in ANP group,the levels of lactulose increased significantly(PR<0.01) with L/M (lactulose/mannnitol) ratio levels 2-12 times higher than that of the control group. In all ANP animals,bacterial translocation(BT)was found,and blood cultures were positive,on day 1 and day 2.In the control group,blood and tissue cultures were negative except for 2 episodes of BT to mesenteric lymphnodes.
出处
《中华普通外科杂志》
CSCD
1997年第4期232-234,共3页
Chinese Journal of General Surgery
