摘要
[Objective] The B cell epitopes for VP73 protein of African swine fever virus was predicted. [ Method] Based on the analysis of the amino acid sequence and the flexible regions of VP73 protein, the B cell epitopes for VP73 protein of African swine fever virus were predicted by method of Kyte-Doolittie, Emini and Jameson-Wolf. [Result] The B cell epitopes were located at or adjacent to the N-terminal No. 11 - 18,26 -48,73 -82,136 - 150,159 - 174,181 - 189,191 - 210,247 - 276,279 - 295,313 - 323 and 382 - 392. [Conclusion] The multi-parameters analytic method was adopted to predict the B cell epitopes for VP73 protein of African swine fever virus, which laid solid foundation for further characterizing the protein of VP73 and researching epitope vaccine.
非洲猪瘟（African Swine Fever，ASF）是由ASF病毒（AS-FV）引起的猪急性、烈性传染病。ASFV可以编码34种以上的结构蛋白。VP73蛋白作为ASFV主要的抗原性结构蛋白，能够诱导机体产生中和抗体，而且抗原性极为稳定，可作为ASFV的主要诊断试剂。笔者利用生物信息学方法，分析了来自Spain 70株的ASFV VP73蛋白的二级结构，并对该蛋白的B细胞表位进行预测，
