摘要
利用花粉管通道技术,以生长在盐碱地的碱蓬总DNA为供体,以1个不耐盐番茄为受体,进行外源DNA直接导入,对转基因番茄D2代分别浇灌1/2和1/4浓度海水进行筛选,获得了抗盐植株。随机抽取20粒抗盐番茄的种子,以碱蓬基因组为探针,通过基因组荧光原位杂交技术进行鉴定,有13株番茄有杂交位点存在,初步证实碱蓬基因存在并整合入抗盐番茄的基因组中。
Using the teehnique of pollen-tube pathway to create new line, the total DNA of suaeda salsa growing in kaline soil was provided as the donor, and salt sensitive tomato was provided as the acceptor. The exogenous DNA had introduced into plant genome by the pollen-tube pathway. In order to gain the salt resistance line, the transgenic tomatos were irrigated with 50% and 25% of the sea water, respectively. Taken 20 seeds randomly, these came form the transgenie tomatoes. Genome fluorescence sltu hybridization technique was used to identify, the DNA of suaeda salsa as probe, The results showed that there were 13 tomatoes had the site of hybridization. It was confiimed that the gene of suaeda salsa was existence and introduced into the tomato genome.
出处
《沈阳农业大学学报》
CAS
CSCD
北大核心
2008年第3期362-364,共3页
Journal of Shenyang Agricultural University
关键词
番茄
花粉管通道
原位杂交
碱蓬
tomato
pollen tube pathway
hybridization in situ
suaeda salsa
