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Subcellular Localizations of AS1 and AS2 Suggest Their Common and Distinct Roles in Plant Development 被引量:1

Subcellular Localizations of AS1 and AS2 Suggest Their Common and Distinct Roles in Plant Development
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摘要 During leaf organogenesis, a critical step for normal leaf primordium initiation is the repression of the class 1 KNOTTED1-like homeobox (KNOX) genes. After leaf primordia are formed, they must establish polarity for normal leaf morphogenesis. Recent studies have led to the identification of a number of genes that participate in the class 1 KNOX gene repression and/or the leaf polarity establishment. ASTMMETRIC LEAVES1 and 2 (AS1 and AS2) are two of these genes, which are critical for both of these two processes. As a first step towards understanding the molecular genetic basis of the ASl-AS2 action, we determined the subcellular Iocalizations of the two proteins in both tobacco BY2 cells and Arabidopsis plants, by fusing them to yellow/cyan fluorescent protein (YFP/CFP). Our data showed that AS1 and AS2 alone were predominantly localized in the nucleolus and the nucleoplasm, respectively. The presence of both AS1 and AS2 proteins in the same interphase cell demonstrated their co-localization in both nucleolus and nucleoplasm. In addition, AS1 alone was able to associate with the condensed chromosome in the metaphase cell. Our data suggest that AS1, AS2 and the ASl-AS2 protein complex may have distinct functions, which are all required for normal plant development. During leaf organogenesis, a critical step for normal leaf primordium initiation is the repression of the class 1 KNOTTED1-like homeobox (KNOX) genes. After leaf primordia are formed, they must establish polarity for normal leaf morphogenesis. Recent studies have led to the identification of a number of genes that participate in the class 1 KNOX gene repression and/or the leaf polarity establishment. ASTMMETRIC LEAVES1 and 2 (AS1 and AS2) are two of these genes, which are critical for both of these two processes. As a first step towards understanding the molecular genetic basis of the ASl-AS2 action, we determined the subcellular Iocalizations of the two proteins in both tobacco BY2 cells and Arabidopsis plants, by fusing them to yellow/cyan fluorescent protein (YFP/CFP). Our data showed that AS1 and AS2 alone were predominantly localized in the nucleolus and the nucleoplasm, respectively. The presence of both AS1 and AS2 proteins in the same interphase cell demonstrated their co-localization in both nucleolus and nucleoplasm. In addition, AS1 alone was able to associate with the condensed chromosome in the metaphase cell. Our data suggest that AS1, AS2 and the ASl-AS2 protein complex may have distinct functions, which are all required for normal plant development.
出处 《Journal of Integrative Plant Biology》 SCIE CAS CSCD 2008年第7期897-905,共9页 植物学报(英文版)
基金 the Scientific and Technological Council Foundation of Shang-hai (04JC14017) the National Talent Training Fund in Basic Research of China (J0630643) to A. Dong the National Natural Science Foundation of China (30630041) the Chinese Academy of Sciences (KSCX2-YW-N-016) to H.Huang
关键词 ARABIDOPSIS asymmetric leaves1/2 leaf development subcellular localization. Arabidopsis asymmetric leaves1/2 leaf development subcellular localization.
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