摘要
目的探讨细胞间粘附分子-3(inter-cellular adhesion molecule-3,ICAM-3)在急性白血病(acute leukemia,AL)中的表达及其在临床疗效、髓外浸润中的意义。方法采用骨髓涂片免疫组织化学法检测了33例急性非淋巴细胞白血病(acute nonlymphoblstic leukemia,ANLL)、21例急性淋巴细胞白血病(acute lymphoblastic leukemia,ALL)患者治疗前后及10例正常对照骨髓单个核细胞ICAM-3的表达率。结果化疗前AL组ICAM-3表达率均显著高于正常对照组,ANLL组与ALL组比较ICAM-3表达率差异无统计学意义(P>0.05),且T-ALL组与B-ALL组ICAM-3表达率差异无统计学意义(P>0.05);ICAM-3的表达与患者发病年龄、性别、就诊时血红蛋白(Hb)、血小板(BPC)等因素无关,与骨髓原始细胞比例呈正相关(r=0.467,P<0.01);外周血白细胞(WBC)≥100×109患者ICAM-3的表达率比WBC<100×109组高(P<0.05);所有AL患者经过一个疗程化疗后ANLL、ALL未缓解组的ICAM-3表达率明显高于缓解组(P<0.01),但缓解组与对照组之间ICAM-3表达率差异无统计学意义(P>0.05);组织浸润组骨髓AL细胞ICAM-3表达率高于无组织浸润组(P<0.01)。结论ICAM-3在AL中的表达明显高于正常对照组,其表达水平的高低可作为AL观察临床疗效及判断预后的指标之一。同时,AL的组织浸润机制可能与细胞间粘附分子表达升高有关。
Objective To explore the expressions of the inter-cellular adhesion molecule-3（ICAM-3） in acute leukemia and to investigate its role in clinical therapeutic efficacy and extramedullary infiltration （EI）. Methods The expressions of ICAM-3 in 33 eases of acute nonlymphoblastie leukemia（ANLL）, 21 eases of acute lymphoblastie leukemia （ALL） and 10 normal bone marrow mononuelear cells were determine by bone marrow smear immunohistochemistry. Results The expression of ICAM-3 in AL patients was significantly higher than that in the controls, and it had no correlation with patients＇ age, gender, Hb and BPC, but had direct correlations with the ratio of primitive bone marrow cells. Also the expression of ICAM-3 was higher in patients whose WBC≥ 100 × 10^9/L than in patients whose WBC 〈 100 × 10^9/L（ P 〈 0.05 ）, and also was much higher in AL patients without complete remission （CR） than in AL patients with CR（ P 〈 0.01 ）, but there were no signifieant differences between patients with CR and the eontrols（P 〉 0.05）. Meanwhile the expression of ICAM-3 was much higher in leukemic cells with EI than that in leukemia cells without EI （ P 〈 0.01 ）. Conclusion ICAM-3 could be used as a helpful marker to assess the therapeutic efficacy and prognosis for AL, and its increase might relate to infiltration of acute leukemic cells.
出处
《山东大学学报（医学版）》
CAS
北大核心
2008年第6期608-611,共4页
Journal of Shandong University:Health Sciences
关键词
急性白血病
细胞间粘附分子-3
髓外浸润
Acute leukemia
Inter-cellular adhesion molecule-3
ExtrameduUary infiltration
