摘要
目的探讨DNA修复基因XRCC3多态性与膀胱癌危险性的关系。方法选择中国汉族人群中膀胱癌患者307例为病例组，316例非肿瘤泌尿系疾病患者为对照组，两组年龄性别构成均衡。以PCR-RFLP技术，检测病例组和对照组的XRCC3（Thr241Met）基因型，比较不同基因型与膀胱癌危险性以及膀胱癌临床特征的关系。结果XRCC3基因型Thr/Thr、Thr/Met和Met／Met在病例组的分布频率分别为87．3％、12．1％和0．6％，在对照组的分布频率为92．4％、7．3％和0．3％。与携带Thr／Thr的个体相比，携带XRCC3变异基因型（Thr／Met和Met／Met）的个体具有更高的患癌风险（OR，1．77；95％CI，1．04-3．02）。XRCC3基因型与吸烟没有交互作用，与膀胱癌的临床类型亦无相关性，但与浅表性膀胱癌的复发关系密切。携带XRCC3变异基因型的浅表性膀胱癌患者具有更高的复发风险（OR，3.08；95％CI，1．13～8．41）。结论XRCC3基因多态性与膀胱癌危险性有关，携带变异基因型的个体易患膀胱癌，而且XRCC3基因有可能成为一个预测膀胱癌复发的指标。
Objective To investigate the relationship between polymorphisms of DNA repair gene XRCC3 and the risk and clinical outcome of bladder cancer. Methods In a hospital based ease-control study for the Chinese population, a total of 307 bladder cancer eases and 316 age- and sex-matched controls with urological non-neoplastic diseases were collected. We analyzed the genotypes of XRCC3 （Thr241Met） by PCR-RFLP and determined their association with bladder cancer risk and clinical outcome. Results The frequency of Thr/Thr, Thr/Met and Met/Met genotype was 87.3%, 12.1% and 0. 6% in eases and was 92.4%, 7.3% and 0.3% in controls. When Thr/Thr served as the reference group, variant genotypes （Thr/Met and Met/Met） had a significant increased risk of bladder cancer（OR, 1.77;95%CI, 1.04-3.02）. Combined with smoking and clinical types, no significant interaction could be found. However, the variant genotypes were associated with an increase of recurrence risk among superficial bladder eaneer（OR,3.08;95%CI, 1.13-8.41）. Conclusions XRCC3 gene polymorphism may be a risk factor for bladder cancer and it may serve as a molecular marker for monitoring bladder cancer recurrence.
出处
《山东大学学报（医学版）》
CAS
北大核心
2008年第6期612-615,共4页
Journal of Shandong University:Health Sciences
基金
北京市自然科学基金资助项目(7052025)
北京市优秀人才专向培养基金资助项目(20061D0300100064)
关键词
多态性
限制性片段长度
膀胱肿瘤
复发
X线交错互补修复基因3
Polymorphism, restriction fragment length
Bladder neoplasms
Recurrence
X-ray repair cross-complementing gene 3
