期刊文献+

脂肪干细胞体外快速扩增实验研究 被引量:1

Experimental Study of Large-Scale Cultivation of Adipose Derived Stem Cells in Vitro
下载PDF
导出
摘要 目的:探索在旋转生物反应器内应用微载体技术快速扩增兔脂肪干细胞的方法。方法:比较应用微载体结合旋转生物反应器(RCSS)进行动态培养与平皿静态培养两种方法中,脂肪干细胞生长曲线、倍增时间和生长周期的情况。结果:实验组RCSS法培养中脂肪干细胞密度可达最初接种的19倍左右,而对照组平皿静态培养仅为6倍余。实验组倍增时间短于对照组,且细胞周期分析显示G2-M期+S期细胞比例高于对照组。结论:利用微载体细胞培养技术可快速地在体外扩增脂肪干细胞。 Objective:To investigate the possibility of quick proliferation of rabbit adipose derived stem cells(ADSCs ) with microcarriers in rotary cell culture system(RCCS). Methods: The ADSCs were culture using two different methods : microcarriers in the RCCS as the experimental group and the static plate as the control group. Growth curve, doubling time and cell cycle were compared between the two groups. Results:Quick growth of ADSCs was observed in the experimental group. The cell growth density and cells in G2-M and S period in RCCS were greater,but the cell doubling time was shorter than those of control group. Conclusion : Microcarrier culture of ADSCs is an easy way that can yield a large quantity of ceils within a short period.
出处 《中国误诊学杂志》 CAS 2008年第21期5047-5049,共3页 Chinese Journal of Misdiagnostics
关键词 细胞培养技术/方法 干细胞/细胞学 脂肪组织/细胞学 细胞 培养的 Cell Culture Techniques/methods Stem Cells/cytology Adipose Tissue/cytology Cells,Cultured
  • 相关文献

参考文献6

  • 1Strem BM, Hicok KC, Zhu M, et al. Multipotential differentiation of adipose tissue derived stem cells[J]. Keio J Med,2005,54(3) : 132- 141.
  • 2Planat-Benard V,Silvestre JS,Cousin B,et al. Plasticity of human adipose lineage cells toward endothelial cells:physiological and t herapeutic perspectives [J].Circulation, 2004,109 (5): 656- 663.
  • 3Wang D,Liu W,Han B,et al. The bioreactor:a powerful tool for large-scale culture of animal cells[J]. Curr Pharm Biotechnol, 2005,6(5):397-403.
  • 4Winter A, Breit S, Parsch D, et al. Cartilage like gene expression in differentiated human stem cell spheroids : a comparison of bone marrow-derived and adipose tissue-derived stromal cells [J]. Arthritis Rheum, 2003,48 (2) : 418-429.
  • 5van-Wezel AL. Growth of cell-strains and primary cells on microcarriers in homogeneous culture[J]. Nature, 1967,216 : 64-65.
  • 6康红军,卢世璧,许文静,张莉,孙明学,袁玫,赵斌,郭全义.人脂肪干细胞结合微载体在生物反应器中向软骨细胞分化[J].中国矫形外科杂志,2007,15(10):773-775. 被引量:10

二级参考文献10

  • 1单建林,许建中,周强,王序全,朱灏,赵敏.微载体技术体外扩增人骨髓间充质干细胞[J].中国矫形外科杂志,2004,12(15):1158-1160. 被引量:6
  • 2Zuk PA,Zhu M,Mizuno H,et al.Multilineage cells from human adipose tissue:implication for cell-based therapies[J].Tissue Engineering,2001,7(2):211 -228.
  • 3Zuk PA,Zhu M,Ashjian P,et al.Human adipose tissue is a source of multipotent stem cells[J].Mol Biol Ceil,2002,13 (12):4279 -4295.
  • 4Planat-Benard V,Silvestre JS,Cousin B,et al.Plasticity of human adipose lineage cells toward endothelial cells:physiological and therapeutic perspectives[J].Circulation,2004,109 (5):656-663.
  • 5Marlovits S,Tichy B,Truppe M,et al.Collagen expression in tissue engineered cartilage of aged human articular chondrocytes in a rotating bioreactor[J].Int J Artif Organs,2003,26 (4):319-330.
  • 6De Ugarte DA,Morizono K,Elbarbary A,et al.Comparison of multilineage cells from human adipose tissue and bone marrow[J].Cells Tissues Organs,2003,174(3):101-109.
  • 7Koch RJ,Gorti GK.Tissue engineering with chondrocytes[J].Facial Plastic Surgery,2002,18(1):59 -68.
  • 8Winter A,Breit S,Parsch D,et al.Cartilage-like gene expression in differentiated human stem cell spheroids:a comparison of bone marrow-derived and adipose tissue-derived stromal stromal cells[J].Arthritis Rheum,2003,48(2):418 -429.
  • 9Saris DB,Sanyal A,An KN,et al.Periosteum reponds to dynamic fluid pressure by proliferating in vitro[J].J Orthop Res,1999,17 (5):668-677.
  • 10Grifiths B.Scale-up of suspension and anchorage-dependent animal cells[J].Mol Biotechnol 2001,7 (3):225-238.

共引文献9

同被引文献19

引证文献1

二级引证文献6

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部