摘要
目的探讨高强度聚焦超声(HIFU)所致的大鼠急性肝损伤后血清炎性因子水平的变化及乌司他丁(UTI)对其的保护效应。方法建立高强度聚焦超声致大鼠急性肝损伤模型,Wistar大鼠24只随机均分为单纯高强度聚焦超声致伤组(H组)、乌司他丁处理组(U组)和空白对照组(D组)。在致伤后1、3、6、12和24h分别检测三组血清中白细胞介素-2(IL-2)、白细胞介素-10(IL-10)和肿瘤坏死因子-α(TNF-α)三种炎性细胞因子的含量。结果与D组比较,H组致伤3h后IL-2和TNF-α开始明显升高,并分别于致伤后6h和12h达到峰值。U组IL-2和TNF-α升高幅度明显低于H组(P<0.01),且于致伤后24h基本恢复正常,而IL-10水平显著高于H组。结论高强度聚焦超声致大鼠急性肝损伤后大鼠机体发生显著炎症反应,乌司他丁能下调致炎因子水平,并可上调抑炎性因子水平,阻断炎症反应,显示出一定的保护效应,改善预后。
Objective To study the protection effect of ulinastatin on the inflammatory reaction of rats after acute liver injury induced by high intensive focused ultrasound （H） . Methods A total of 24 Wistar rats were randomly divided into simple HIFU liver injury group （H）, ulinastatin-treated group （U） and control group （D）. The serum contents of interleukin （IL）-2, IL-10, and tumor necrosis factor-α （TNF-α） were determined at 1,3,6,12, and 24 h after acute liver injury by HIFU. Results Compared with group D, the levels of serum IL-2 and TNF-α were significantly higher at 3 h and reaehed the peak at 6 and 12 h after acute liver injury by HIFU in group H. In group UTI, the levels of serum IL-2 and TNF-α were significantly lower than those in group H at the same time points （P〈0. 01） and return to normal level at 24 h. The levels of serum IL-10 in group UTI were signifieantly higher than those in group H （P〈0.01）. Conclusion UTI can decrease the production of inflammatory factors and upregulate the serum levels of anti-inflammatory factors, retard the development and progression of acute liver injury by HIFU.
出处
《临床麻醉学杂志》
CAS
CSCD
2008年第6期518-520,共3页
Journal of Clinical Anesthesiology
关键词
高强度聚焦超声
急性肝损伤
乌司他丁
炎性因子
High intensive focused ultrasound
Acute liver injury
Ulinastatin
Inflammatory faetor
