摘要
从大粒型小麦品种豫教2号中克隆出调控小麦ADPG焦磷酸化酶(AGPase)胞质型大亚基基因(LSUI)cDNA全长。在花后15d的小麦植株中,通过半定量PCR法,发现LSUI基因在叶、籽粒、叶鞘和颖壳中表达量较高,但在根和茎中表达量较低,表明在绿色光合功能器官和淀粉合成器官中,LSUI基因量表达较高。比较了LSUI基因在千粒重不同的豫教2号和兰考矮早八两小麦品种籽粒发育过程中的表达差异后,发现在籽粒形成期,LSUI基因在两品种籽粒内表达相似,但在籽粒灌浆期和成熟期间,LSUI基因在千粒重较高的豫教2号内表达量明显高于千粒重较低的兰考矮早八,这个表达差异与两品种间淀粉积累差异趋势相似,表明LSUI基因的表达量可能与淀粉的积累量密切相关。
The 1941-bp full cDNA sequence （GenBank No. DQ839506） of LSU I, the cytoplasmic large subunit gene I of adenosine 5＇ diphosphate glucose pyrophosphorylase （AGPase）, was cloned from a high 1000-grain weight wheat （Triticum aestivum L.） cultivar Yujiao 2. Semi-quantitive PCR analysis showed that LSU I expressed higher in leaves, glumes, sheaths, and grain than in roots and stems, indicating that LSU I expressed actively in photosynthetic and starch synthesis-related organs. During the primal period of grain development （5–10 d after thesis）, there was no difference in transcript levels of LSU I gene between Yujiao 2 and Lankao Aizao 8 （low 1000-grain weight）, but the transcript amounts in grains of Yujiao 2 was markedly higher than that of Lankao Aizao 8 at grain filling and maturity stages. The changes of LSU I gene expression were similar to the trend of starch accumulation in the two cultivars, suggesting that LSU I gene may be associated with starch accumulation in common wheat.
出处
《作物学报》
CAS
CSCD
北大核心
2008年第7期1290-1293,共4页
Acta Agronomica Sinica
基金
中国博士后科学基金项目(20060390773)
河南省教育厅自然科学基金项目(2006210007)
