摘要
目的:应用99mTc-MDP放射性骨显像观察复合兔骨髓基质细胞的纳米羟基磷灰石/胶原(BMSCs+nHAC)修复兔下颌骨缺损的能力。方法:兔下颌骨体部范围为15mm×15mm的全层骨缺损分别采取相应方法修复:A组,BMSCs+nHAC修复;B组,nHAC修复;C组,空白对照组,缺损不做修复。术后4、8、12周分别通过放射性核素骨显像进行监测。结果:术后4周,A组核素浓聚程度明显高于B组、C组,差异具有统计学意义,B组核素浓聚程度亦高于C组,差异具有统计学意义;术后8周,A组核素浓聚程度明显高于B组、C组,差异具有统计学意义,B组核素浓聚程度与C组无显著差异;术后12周,3组核素浓聚程度无显著性差异。结论:BMSCs+nHAC修复下颌骨缺损与单独使用nHAC相比具有较好的成骨能力和血管化程度。
Objective： To observe the rabbit mandibular defect repairing ability of nano-hydroxyapatite/collagen composed of rabbit bone marrow stromal cells （BMSCs＋nHAC） using 99mTc-MDP ECT. Methods： The size of 15mm×15mm full-thickness defect in rabbit mandibular was repaired by following methods： group A： repaired with BMSCs and nHAC; group B：repaired with nHAC; group C： control group, with no repair to defect. 99mTc -MDP ECT was used to monitor at 4, 8, 12 weeks after operation. Results： 4 weeks after operation, the uptake of 99mTc-MDP in group A was higher than that of group B and group C（P〈0.05 ）. The uptake of 99mTc-MDP in group B was higher than that of group C（P〈0.05）. 8 weeks after operation, the uptake of 99mTc-MDP in group A was higher than that of group B and group C（P 〈0.05）, but there was no significant difference between group B and group C.12 weeks after operation, there were no significant differences between three groups. Conclusion： Compared with using nHAC to repair mandibular defect, using nHAC composed of rabbit BMSCs was better for bone formation and vascularization.
出处
《口腔医学研究》
CAS
CSCD
2008年第3期278-281,共4页
Journal of Oral Science Research
