摘要
背景与目的Ezrin蛋白为膜细胞骨架连接蛋白,近年来研究发现ezrin蛋白在多种肿瘤的发展、浸润和转移过程中扮演重要角色。本研究目的是探讨ezrin在肺癌组织及肺癌细胞系中的作用及其临床意义。方法采用免疫组化二步法检测75例肺癌及16例正常肺组织标本中ezrin蛋白的表达,结合肺癌的临床病理特点进行分析。同时应用RT-PCR及激光扫描共聚焦显微镜检测肺癌细胞系的ezrin基因及蛋白水平的表达情况。结果肺癌组织中ezrin的阳性表达率(77.3%)较正常肺组织(100%)有所降低,两者比较差异有统计学意义(P<0.05)。ezrin表达下降与肺癌患者性别、年龄、肿瘤大小、病理类型、组织分化程度、临床分期等无关,与有无区域淋巴结及远处转移有关。另外,ezrin表达在肺癌有从胞膜向胞浆移位倾向,这与体外实验结果一致。结论肺癌组织及肺癌细胞系中ezrin的表达下降,其从胞膜向胞浆的移位可能参与肺癌的发生发展。
Background and objective Metastasis is the main cause of cancer related mortality, and identification of genes involved in tumor metastasis is important for effective therapies. The membrane cytoskeletal crosslinker participated in several functions including cell adhersion, motility and cell survival, and there is increasing evidence that it regulates tumor progression. However, the role played by ezrin in lung cancer metastasis has not been clearly delineated. The aims of this study are to investigate the ezrin expression pattern in human lung carcinoma and the correlation with clinicopathologic characteristics. Methods Ezrin expression was detected by twostep immunohistochemical staining technique in tumor tissues from 75 lung cancer cases and in normal lung tissues from 16 cases with benign disease and analyzed by the lung cancer clinicopathologic characteristics. The gene and protein level expression of Ezrin in lung cancer cell lines was also detected by Confocal Laser Scanning Microscope （CLSM） and RT-PCR. Results The positive incidence of ezrin expression （77.3%） was significantly lower in lung cancer tissues than that in normal tissues （100%） （P〈0.05）, and the down-regulated of ezrin expression was significantly correlated with lymph node metastasis and distant metastasis （P〈0.05） and was not correlated with gender, age, tumor size, pathological type, the degree of differentiation of tissue and clinical stage.Otherwise, the subcellular redistribution of ezrin from cell membrane to cell plasma was significantly correlated with lymph node metastasis （P〈 0.05）, in consistance with in vitro experimental result. High metastasis cell line BE1 had the lowest expression of ezrin under CLSM, similar to RT-PCR, and ezrin was mainly located in cell plasma under CLSM. Conclusion Ezrin expression is down-regulated in lung cancer tissue and lung cancer cell line, and the locational change from cell membrane to cell plasma may be associated with the oncogenesis and development of lung cancer.
出处
《中国肺癌杂志》
CAS
2008年第1期67-73,共7页
Chinese Journal of Lung Cancer
