摘要
背景与目的已有的研究显示:p120ctn(p120 catenin)与肿瘤的发生密切相关,在保持细胞黏附中起着重要作用,本研究通过观察p120ctn各亚型在肺鳞癌、腺癌和相应的癌旁肺组织中蛋白表达量的变化,探讨p120ctn亚型与各临床病理因素的关系。方法应用免疫荧光和Western Blot方法检测肺鳞癌、腺癌及对应癌旁正常肺组织中p120ctn各亚型的表达情况。结果与正常肺组织相比,p120ctn在肺癌细胞膜表达减弱,呈现胞膜荧光不连续或缺失,胞浆内存在p120ctn的蓄积。正常肺组织中p120ctn总蛋白量明显高于肺癌组织(P<0.001)。p120ctn蛋白以亚型1(120KD)和亚型3(100KD)表达为主,而在肺癌组织则出现1、3亚型表达缺失或减弱(P=0.001,P<0.001)。并且亚型3的这种缺失或减弱的现象与肺癌的淋巴结转移负相关(P=0.005)。结论p120ctn亚型1和3的蛋白表达的减弱或缺失是肺癌的普遍现象,且这种现象与淋巴结的转移相关。
Background and Objective It has been proven that p120ctn plaied an important role in cell adhesion and correlates with tumorigenesis significantly, the aim of this work is to investigate the expression of p120ctn （p120 eatenin） isoform proteins in lung squamous cell carcinomas and adenocarcinomas, and analyze the correlations between p120ctn and clinicopathological parameters. Methods Immunofluorescence and Westren Blot were used to detect the expression of p120ctn isoforms in lung cancer tissues and the corresponding normal lung tissues. Results Compared to normal epithelium of lung, lung cancer tissues show reduction or absence of membrane expression or cytoplasmic accumulation of p120ctn. In normal lung tissues, the total levels of p120ctn proteins were significantly higher than that in lung cancer tissues （P〈0.001）. The isoform 1 （120KD） and isoform 3 （100KD） proteins were the major isoforms of p120ctn expressed in normal lung tissues, which were significantly reduced in lung cancer samples （P=0.001 and P〈0.001, respectively）. Furthermore, the changes in isoform 3 protein levels was negatively associated with lymph node metastasis （P=0.005）. Conclusion Reductions of p120ctn isoform 1 and 3 is a common phenomenon in lung cancer tissues and may play a role in metastasis progression in human lung cancer.
出处
《中国肺癌杂志》
CAS
2008年第2期189-193,共5页
Chinese Journal of Lung Cancer
基金
国家自然科学基金(No.30470764
No.30670917)资助~~
