摘要
为探讨豚鼠耳蜗核一氧化氮合酶（ｎｉｔｒｉｃｏｘｉｄｅｓｙｎｔｈａｓｅ，ＮＯＳ）神经元在白噪声损伤过程中可能的作用，采用硫辛酰胺脱氢酶（ＮＡＤＰＨ－ｄ）组织化学方法及半定量多聚酶链反应（ＰＣＲ）技术，研究白噪声暴露后豚鼠耳蜗核ＮＯＳ神经元及ＮＯＳｍＲＮＡ含量的变化以及与听阈的关系。结果表明，噪声暴露后耳蜗核ＮＯＳ阳性神经元的数量及染色强度明显增加，２周达高峰，３～４周持续高表达，５周有所恢复，但仍高于正常水平。耳蜗核ＮＯＳｍＲＮＡ含量的变化规律与形态学观察结果一致，２周组ＮＯＳｍＲＮＡ含量最高，是正常的５．０２倍。噪声暴露后ＡＢＲ阈移与耳蜗核ＮＯＳｍＲＮＡ含量呈正相关（ｒ＝０．９６５５，Ｐ＜０．０１）。提示耳蜗核ＮＯＳ阳性神经元可能参与了耳蜗神经损伤修复的调节，ＮＯＳ基因的高表达是噪声性聋发病机制中不可忽视的重要因素之一。
NADPH diaphorase( NADPH d) histochemistry method and semiquantitative polymerase chain reaction ( PCR) technology were adopted to study nitric oxide synthase (NOS) neurons and to investigate the content change of NOS mRNA and the relationship between it and auditory threshold and to explore the possible role of NOS neurons in cochlear nuclei of guinea pigs after exposed to white noise. The results showed that the quantity and staining intensity of positive NOS neurons in cochlear nuclei increased obviously after exposed to noise. It reached the peak 2 week later and remained 5 week than normal. The regulation of NOS mRNA was consistent with that of morphological observation. NOS mRNA content in 2w group was the highest and was 4.02 times higher than normal. After exposed to noise, ABR threshold shift was in positive correlation with NOS mRNA content in cochlear nuclei( r =0.9655, P <0. 01) . It is suggested that positive NOS neurons in cochlear nuclei might be involved in controlling injury and repair of cochlear nerves. The high expression of NOS gene might be an important factor in pathogenic mechanism of noise deafness.
出处
《中华耳鼻咽喉科杂志》
CSCD
1997年第5期268-272,共5页
Chinese Journal of Otorhinolaryngology
基金
国家自然科学青年基金
关键词
噪声
一氧化氮
蜗神经核
基因表达
PCR
Noise
Nitric oxide
Cochlear nucleus
Gene expression
Polymerase chain reaction
