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放烧复合伤后白细胞介素-3基因的表达 被引量:3

Changes of interleukin 3 expression after combined radiation burn injury in mice
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摘要 为了进一步探讨放射损伤和放烧复合伤引起造血功能障碍的机理,研究了作为造血调控的重要细胞因子——白细胞介素-3(IL-3)基因在放射损伤、烧伤、放烧复合伤后不同时相点基因表达的变化。方法mRNA抽提、分子杂交、细胞培养、IL-3生物活性测定、3H-TdR参与实验等生物技术方法。结果放射损伤后IL-3基因的表达受到明显抑制,照射后第3,14天表达的IL-3mRNA减少了77%,21%;IL-3蛋白减少了83%,36%。烧伤对IL-3基因表达的影响呈双相性,烧伤后第3天IL-3表达受到抑制;烧伤后第14天IL-3的表达增强;放烧复合伤后IL-3的表达受到抑制,其抑制程度介于放射损伤与烧伤之间,各观察组IL-3基因表达的变化与骨髓有核细胞数的变化呈平行关系。结论放射损伤、放烧复合伤后IL-3基因表达被抑制可能是急性放射病引起造血功能障碍的原因之一。 Objective\ To study the changes of IL 3 expression after 5Gy irradiation, 30% total body surface area burn and combined radiation burn injury in mice. Methods\ Northern dot blotting, production of mRNA, cell culture, assay of IL 3 biological activity, etc. Results\ The IL 3 mRNA was markedly decreased by 77% and 21% on the 3rd day and the 14th day after irradiation, respectively. The IL 3 protein was decreased by 83% and 36% on 3rd and 14th day, respectively. There was a significant inhibition of IL 3 expression on the 3rd day after simple burn and slight increase of IL 3 expression on the 14th day. In groups with combined injury, IL 3 was more than that in the irradiated groups but less than that in the groups with burn injury. The changes of IL 3 expression were parallel with the changes of bone marrow cells. Conclusion\ Inhibition of IL 3 expression is one of the reasons of hematopoietic failure caused by radiation and combined radiation burn injury in mice.
出处 《中华放射医学与防护杂志》 CAS CSCD 北大核心 1997年第4期230-232,共3页 Chinese Journal of Radiological Medicine and Protection
基金 国家自然科学基金
关键词 放射损伤 烧伤 放烧复合伤 白细胞介素-3 Radiation injury\ Burn\ Combined radiation burn injury\ Interleukin 3\ Gene
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参考文献6

  • 1叶治家,第三军医大学学报,1996年,18卷,19页
  • 2叶治家,国外医学临床生物化学与检验学分册,1995年,16卷,106页
  • 3吴炳义,第二军医大学学报,1993年,6卷,535页
  • 4周度金,生物化学杂志,1987年,3卷,25页
  • 5郑景熙,中华放射医学与防护杂志,1987年,7卷,17页
  • 6罗成基,中华放射医学与防护杂志,1984年,4卷,2期,18页

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