摘要
目的:观察逆灸对心肌缺血再灌注损伤大鼠HSP70表达的影响,并与缺血预处理的效果进行比较,探讨逆灸有效性和对心肌的保护机制。方法:①选用健康Wistar大鼠30只,雄性,体重(250±20)g,随机分成5组:正常对照组、假手术组、心肌缺血再灌注组(IR组)、心肌缺血预处理组(IP组)、逆灸组,每组6只。②免疫组织化学法检测大鼠心肌组织HSP70表达。③计量资料差异比较采用spss16.0软件统计。结果:逆灸"内关""膻中"穴可明显增加心肌缺血再灌注损伤型大鼠心肌组织中HSP70的表达,与IR组比较差异显著(P<0.05),且与IP组无显著差异(P>0.05)。结论:逆灸与缺血预处理均可增加HSP70的表达,减轻心肌缺血再灌注对心肌造成的损伤,丰富了"治未病"理论。
Objective：To observe the influence of HSP70 by preventive moxibustion on isehemia reperfusion rats, and compare with the effect of Ischemic Precondition, discuss the effectiveness and protection mechanism of preventive moxibustion. Methods：①30 healthy male Wistar rats were randomly divided into 5 groups：A control group, a false surgery group, a ischemia reperfusion group,a ischemic precondition group and a preventive moxibustlon group.②Immunohistochemical test on HSPT0 of myocardial tissue of big rats. ③Statistical measurement data of differences comparison was Aanalyzed by Spss. Results ： preventive moxibustion on Neiguan and Tanzhong can significantly increase the expression HSP70 in the tissue of ischemia reperfusion rats,which varies considerably compared with the IR group（P 〈 0. 05） and no significant variation compared with the IP group （ P 〉 0. 05 ）. Cortclusion ： preventive moxibustion and Ischemic Precondition can increase the expression of HSP70, and reduce the myocardial injury of ischemia reperfusion. The manipulation enriches the theory of＂treatment before being sick＂.
出处
《中华中医药学刊》
CAS
2008年第7期1382-1383,共2页
Chinese Archives of Traditional Chinese Medicine
基金
黑龙江研究生创新科研基金资助项目(YJSCX2007-0169HLJ)
关键词
心肌缺血
逆灸
HSPTO
缺血预处理
myocardial ischemia
preventive moxibustion
heat shock protein 70 （HSP70）
ischemic precondition
