摘要
研究CD4、CD8在肺气虚证大鼠肺和皮肤中的表达,旨在探讨"肺主皮毛"的免疫学基础。采用气熏法制造肺气虚证模型,实验设为模型组和正常对照组。采用S-P免疫组化方法检测皮肤和肺组织中CD4和CD8的表达。结果表明肺气虚证大鼠肺脏和皮肤CD8+细胞均升高(P<0.05);皮肤CD4+细胞发生迁移,但数量没有变化(P>0.05),肺CD4+细胞数量没有变化,二者免疫功能均下降,从免疫学角度证实肺主皮毛理论具有一致性。
Objective ：To study the expression of CD4 CDs in the lung and skin of rats with lung Qi -deficiency syndrome, to demonstrate the consistency on the immunological experiments for the theoretic of ＂the lung is in charge of the skin＂. Methods ：To establish lung Qi - deficiency syndrome model with fuming gating. The rats were divided into the norreal control group and the model group. CD4 and CDs were examined by the S - P immunohistochemical method. Results ： CD8^＋ cell of lung and skin of rats with lung Qi - deficiency syndrome were higher than the control group （ P 〈 0.05 ） ; CD4^＋ cell of skin were moved from dermis to epidermis, but the quantity maintained the same. CD4^＋ cell of the lung maintained the same,immune function of two tissues decreased. Conclusion ：The theoretic of the lung is in charge of the skin were identical in the immune sapect.
出处
《中华中医药学刊》
CAS
2008年第7期1538-1540,共3页
Chinese Archives of Traditional Chinese Medicine
基金
内蒙古医学院重大课题项目(NY2003ZD006)
