摘要
目的通过观察碘硒对大鼠腹腔巨噬细胞抗原提呈作用的影响，初步探讨两者对自身免疫性甲状腺疾病发病的影响及其免疫学机制。方法选用雌性Lewis大鼠20只，根据随机抽样的原则，分为4组：低硒适碘组（LSnNI），低硒高碘组（LSeHI），适硒适碘组（NSeNI），适硒高碘组（NSeHI）。各组用人工合成的低硒低碘饲料和饮用含不同浓度硒或碘的去离子水（用KIO3和Na2SeO3配置）喂养3个月。制备各组大鼠腹腔巨噬细胞及卵白蛋白（OVA）致敏的T细胞，将两者共同培养，进行抗原提呈实验，采用ELISA方法测定上清中IL-2水平；采用RT-PCR方法检测各组脾细胞共刺激分子CD。mRNA的表达水平。结果NSeHI组培养上清中IL-2水平为（43．22±3．27）pg／ml明显高于NseNI组IL-2水平（25．74±2．45）pg／ml。LseNI组IL-2水平为（15．79±2．13）pg／ml明显低于NseNI组。NseHl组大鼠脾细胞CD86mRNA表达水平（CD86／B．aetin：0．52±0．10）明显高于NseNI组（CD86／B-actin：0．35±0．04）。结论高碘使巨噬细胞的抗原提呈作用呈现高于正常状态，成为诱发甲状腺自身免疫发生的一个重要的因素。低硒可使大鼠腹腔巨噬细胞对OVA抗原识别和提呈作用减弱，维持免疫自稳机制失调，可能也是构成诱发自身免疫病的一个因素。
Objective To observe the effects of iodine/selenium on the function of antigen presentation of peritoneal macrophages in rats and explore the immunological mechanisms of iodine/ selenium＇s role in pathogenesis of autoimmune thyroid diseases （AITD）. Methods Female Lewis rats were randomly divided into four groups including①low selenium and normal iodine group（ LseN I ）②low selenium and high iodine group （ LseH I ）③normal selenium and normal iodine group（ NseN I ）④normal selenium and high iodine group （ NseH I ）- All rats were fed by a special diet with lower selenium and iodine in it and drunk ion-free water containing different levels of iodine and selenium for 3 months. Peritoneal macrophages of each group and OVA allergized T cells were prepared and cultured together. Then the function of antigen presentation were estimated by detecting the levels of IL-2 in the culture supernatant. The levels of the expression of co-stimulator CD86 in the spleen of each group were determined by RT-PCR. Results The level of IL-2 in the supernatant in NseH I （43.22 ±3.27） pg/ml was much stronger than NseN I [ the level of IL-2was （25.74 ±2.45）pg/ml, P〈0.05].The level of IL-2 in LseNI （15.79 ±2.13） pg/ml was significantly lower than Nse N I （ P 〈 0. 05 ） . The expression of CD86 mRNA in NseHI （ CD86/β-actin： 0. 52 ±0. 10）were higher than NsNI （ CD86/β-actin：0. 35 ±0. 04） ,P 〈0. 05. Conclusions High iodine could promote the presentation function of macrophages to a higher state than normal. Therefore, high iodine intake might become an importantly inducing factor in thyroid autoimmunity. Low selenium could weaken the ability of recognizing and presenting OVA antigen of peritoneal macrophages which might destroy immunological homeostasis and thus the low selenium intake might also become an inducer of AITD.
出处
《中华预防医学杂志》
CAS
CSCD
北大核心
2008年第7期485-488,共4页
Chinese Journal of Preventive Medicine
基金
天津市科技发展计划项目（05YFGDSF02700）
关键词
碘
硒
抗原提呈
Iodine
Selenium
Antigen presentation
