摘要
以鳗弧菌（Vibrio anguillarum）toxR基因内366～571bp之间的基因序列为靶序列，采用PCR法制备对鳗弧菌特异性的地高辛标记DNA探针，探针长度为206bp。选取2株鳗弧菌和8株与鳗弧菌亲缘关系非常接近的常见水产动物致病弧菌，通过斑点杂交法对制备的探针进行特异性检测，结果显示该探针对鳗弧菌杂交阳性，而与弧菌属的其它8株细菌均无交叉反应。这表明该地高辛标记探针具有很强的特异性，它能够很好地对鳗弧菌感染进行检测。
A digoxigenin labeled DNA probe was produced by PCR method using toxR gene between 366 bp and 571 bp of Vibrio anguillarum as a target. The length of probe was 206 bp. Together with two V. anguillarum strains, ten pathogenic vibrio strains were chosen to detect the specificity of probe for V. anguillarum by dot blot hybridization. Result showed that the probe was able to hybridize with V. anguillarum. And no hybridization signals were observed using other eight vibrio strains most close to V. anguillarum, which demonstrated that the probe had strong specificity and had high value in detecting V. anguillarum.
出处
《淮海工学院学报（自然科学版）》
CAS
2008年第2期66-69,共4页
Journal of Huaihai Institute of Technology:Natural Sciences Edition
基金
淮海工学院自然科学基金资助项目(Z2006036)
关键词
鳗弧菌
PCR法
地高辛标记探针
斑点杂交
V. anguillarum
PCR
digoxigenin labeled DNA probe
dot blot hybridization
