摘要
目的:探讨脱氢表雄酮(DHEA)对实验性自身免疫性神经炎(EAN)大鼠细胞免疫反应的影响。方法:21只Lewis大鼠随机分为DHEA 0.5mg治疗组、2mg治疗组和对照组,治疗组于注射牛周围神经髓磷脂(BPM)抗原乳剂后第5天开始每日皮下注射DHEA,对照组皮下注射相同体积的DHEA溶媒。疾病高峰期检查坐骨神经IFN-γ、TNF-α阳性反应细胞,检测引流淋巴结和脾脏单个核细胞增殖反应,检测培养上清TNF-α、IFN-γ、IL-10含量。结果:两种剂量DHEA治疗组均能减少坐骨神经IFN-γ、TNF-α阳性反应细胞数目(P<0.05),抑制BPM刺激T淋巴细胞增殖(P<0.05),降低培养上清中IFN-γ、TNF-α水平(P<0.05)。各组间IL-10水平差异无统计学意义(P>0.05)。结论:DHEA可抑制EAN大鼠自身反应性T淋巴细胞增殖和促炎性细胞因子过度表达,抑制细胞免疫反应。
AIM： To explore the effects of dehydroepiandrosterone（DHEA） on cellular immune response in experimental autoimmune neuritis（EAN）.METHODS： 21 female Lewis rats were randomly divided into DHEA 0.5 mg treatment group,2 mg treatment group and control group（n=7）.Treatment groups were subcutaneously injected every day with DHEA and the control group with the same volume of DHEA dissolvent from day 5 post immunization（p.i.） with bovine peripheral myelin（BPM）in Freund＇s complete adjuvant（CFA）.The effects were assessed in terms of the number of IFN-γ,TNF-α positive cells in sciatic nerve sections,T-cell proliferation and inflammatory cytokines（IFN-γ,TNF-α and IL-10） synthesis by draining lymph node and spleen cells at the height of clinical EAN.RESULTS： Rats treated with DHEA at different doses displayed significant decreases in numbers of IFN-γ and TNF-α expressing cells in the PNS（P〈0.05）,BPM-stimulated T cell proliferation（P〈0.05） and IFN-γ,TNF-α secretion in the draining lymph node and spleen（P〈0.05） compared to control group.No significant difference of supernatant IL-10 was found among the different groups（P〉0.05）.CONCLUSION： Administration with exogenous DHEA inhabits cellular immune response by suppressing the proliferation of autoreactive T-cell and production of pro-inflammatory cytokines in Lewis rats with EAN.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2008年第8期760-763,共4页
Chinese Journal of Cellular and Molecular Immunology
基金
山东自然科学基金资助项目(Y2007C169)
关键词
脱氢表雄酮
神经炎
实验性自身免疫性
细胞免疫
dehydroepiandrosterone
neuritis,experimental autoimmune
cellular immunity
