摘要
背景:选择组织相容性良好的聚合物材料作为心脏涂层支架的材料可以促进内皮细胞生长,抑制平滑肌细胞过度生长,减少局部炎症反应,有助于支架术后内皮损伤的早期修复,并减少支架局部血栓形成。目的:实验拟观察316L不锈钢经丝素肽/壳聚糖聚合物涂层后的血液相容性。设计、时间及地点:观察性实验,于2006-07/2007-07在中国科学院上海生命科学研究院植物生理生态研究所完成。材料:医用316L不锈钢片为张家港先科医疗器械有限公司产品;丝素肽为中国科学院上海植物生态研究所产品;壳聚糖为浙江省玉环县海洋生物化学有限公司产品。方法:通过静电自组装的方法,将丝素肽和壳聚糖涂在316L不锈钢表面形成聚合物膜。主要观察指标:测定不锈钢涂层前后表面的动态凝血时间、扫描电镜观察涂层前后的不锈钢表面抗血小板作用、测定涂层不锈钢的溶血率。结果:①不锈钢表面经过聚合物涂层后,凝血时间明显较未涂层的不锈钢时间延长。②裸316L不锈钢表面黏附的血小板数量较多,血小板变扁平,看不出明显的伪足状态,紧贴在材料的表面;丝素肽/壳聚糖聚合物不锈钢涂层表面也有一定数量的血小板黏附,血小板虽有伪足伸出,但是聚集密度和形态变化程度明显小于裸不锈钢表面黏附的血小板。③涂层不锈钢溶血率<5%,提示丝素肽/壳聚糖聚合物涂层不锈钢无溶血作用结论:与裸316L不锈钢相比,丝素肽/壳聚糖聚合物涂层延长了动态凝血时间,减轻了血小板黏附和变形的程度,提高了316L不锈钢基体的血液相容性。
BACKGROUND： Applying polymer materials with good histocompatibility as the coating scaffolds of the hearts, may promote the growth of endothelial cells, inhibit the excessive growth of smooth muscle cells and reduce the local inflammatory reaction. Thus polymer materials are effective to improve the repair of endothelial injury and reduce the occurrence of thrombosis.
OBJECTIVE： To observe the blood compatibility of 316 L stainless steel with silk peptide/chitosan polymeric coating membrane. DESIGN, TIME AND SETTING： An observation experiment was performed at Institute of Plant Physiology and Ecology, Shanghai Institute of Life Sciences, Chinese Academy of Sciences between July 2006 and July 2007.
MATERIALS： 316L stainless steel was produced by Zhangjiagang Xianke Medical Apparatus Co., Ltd （China）; Silk peptide was produced by Institute of Plant Physiology and Ecology, Shanghai Institute of Life Sciences, Chinese Academy of Sciences （China）; Chitosan was produced by Zhejiang Province Yuhuan Marine Biochemistry Co., Ltd （China）.
METHODS： By means of electrostatic self-assembly method, a kind of polymeric membrane was prepared with silk peptide and chitosan and then applied on the surface of 316L stainless steel.
MAIN OUTCOME MEASURES： The dynamic clotting time, platelet adherence through scanning electron microscope and hemolysis ratio of the stainless steel were all detected before and after coating to test the blood compatibility of this polymeric membrane. RESULTS： The dynamic clotting time for 316L stainless steel coated with the polymer of silk peptide and chitosan remarkably prolonged compared to that with no coating. The adherence experience showed there were more blood platelets sticking on the surface of the bare stainless steel, and the platelets deformed; While some platelets were also found on the surface of the coated 316L, but the platelet deformation and the density of aggregation were obviously less than the bare stainless steel. The hemolysis rate of the coated steel was less than 5%, which suggested the silk peptide/chitosan polymeric coating was avirulent.
CONCLUSION： Compared to the bare 316L stainless steel, the silk peptide/chitosan polymeric coating prolongs the activated coagulation time, alleviates the adherence and distortion of platelets, and enhances the blood compatibility of 316L base.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2008年第23期4461-4464,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
