摘要
背景：海洋胶原肽是相对分子质量在1000以下的肽为主要成分的粉末状低聚肽产品，由2～6个氨基酸组成，体外实验证实其具有抗氧化等生物活性。目的：观察海洋胶原肽对2型糖尿病大鼠血清中过氧化应激标志物表达的影响。设计、时间及地点：随机对照动物实验，于2006—10／2007-06在深圳市疾病预防控制中心动物实验室完成。材料：海洋胶原肽由中国食品发酵工业研究院赠送。雄性SD大鼠36只，随机取6只为正常对照组，给予基础饲料，其余30只高脂饲料喂养4周后再小剂量分次腹腔注射链脲佐菌素诱导2型糖尿病模型，26只造模成功。干预：取24只模型鼠随机分为4组，海洋胶原肽0．75，1．5，3．0g／（kg·d）组给予相应剂量的海洋胶原肽灌胃4周，糖尿病组给予消毒后的自来水作安慰剂灌胃4周。正常对照组也灌胃自来水4周。主要观察指标：干预前及干预4周后各组大鼠的血糖水平，并检测干预4周后各组大鼠血清中超氧化物歧化酶、丙二醛、还原型谷胱甘肽及NO等过氧化应激指标变化。结果：干预4周后，海洋胶原肽各剂量组血糖虽然低于糖尿病组，但差异无显著性意义（P〉0．05）。与正常对照组比较，各组血清超氧化物歧化酶、还原型谷胱甘肽水平下降，丙二醛、NO浓度上升（P〈0．01，0．05）；海洋胶原肽3．0，1．5g／（kg·d）组血清超氧化物歧化酶、还原型谷胱甘肽水平高于糖尿病组，丙二醛、NO浓度低于糖尿病组（P〈0．01，0．05）。结论：较高剂量[≥1．5∥（kg·d）]的海洋胶原肽可以改善高血糖和高脂膳食负荷的2型糖尿病大鼠体内过氧化应激标志的表达。其对于血糖的干预效果分析还需要加大样本量和干预时间作进一步的探讨。
BACKGROUND： Marine collagen peptide is a kind of oligopeptide powder at the relative molecular mass of less than 1 000, comprising 2 6 amino acids. Its antioxidation property has been proved in vitro.
OBJECTIVE： To study the effect of marine collagen peptide on the expression of overoxidation stress markers in the blood serum of rats with type 2 diabetes.
DESIGN, TIME AND SETTING： A randomized control animal study was carried out from October 2006 to June 2007 at the Laboratory of Animal Experiment in Shenzhen Center for Disease Control and Prevention （Shenzhen, Guangdong, China）.
MATERIALS： Marine collagen peptide was offered by the China National Research Institute of Food and Fermentation Industry. Thirty-six male SD rats were assigned into 6 controls and 30 models. Normal control group fed with basal diet, while 26 of other 30 rats were successfully induced the model of type 2 diabetes by intraperitoneal injection of streptozotocin.
METHODS： Twenty-four rats with type 2 diabetes were randomly divided into four groups, namely marine collagen peptide low dose group （0.75 g/kg ·d）, medium dose group （1.5 g/kg·d）, high dose group （3.0 g/kg ·d） and diabetes control group. They were all processed into intragastric administration of tap water for 4 weeks.
MAIN OUTCOME MEASURES： Blood glucose of rats before intervention and at four weeks after intervention. Blood level of superoxide dismutase （SOD）, malondialdehyde （MDA）, reduced glutathione hormone （GSH） and NO of rats at four weeks after intervention. RESULTS： There were no significant differences among three marine collagen peptide groups on the blood glucose （P 〉 0.05）. Compared to normal control group, the levels of SOD and GSH of all groups were significantly lowered, while the levels of MDA and NO were significantly enhanced （P 〈 0.01, 0.05）; Compared to the diabetes control group, the SOD and GSH levels of marine collagen peptide middle and high dose groups were significantly increased, while the MDA and NO levels were significantly lowered （P 〈 0.01,0.05）. CONCLUSION： Higher dosage of marine collagen peptide （/〉 1.5 g/kg · d） may have a regulating effect on the expression of overoxidation markers in rats with type 2 diabetes. The exact effect of marine collagen peptide on fasting blood glucose deserves further study of large scale samples and intervention time.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2008年第23期4469-4472,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
国家科技支撑计划资助项目(2006BAD27B01)~~
