摘要
本研究利用皖西白鹅为父本与四川白鹅(母本)进行杂交,屠宰测定了123只杂种鹅的胴体性状、羽绒性状和肉质性状,运用因子分析从这些性状中提取了12个公共因子;采用4种内切酶Hinf I、EarI、PstⅠ和PvuⅡ检测了鹅黑素皮质素受体-4(MC4R)基因编码区的RFLP,并分析了RFLP与鹅重要经济性状之间的关联性。结果显示,试验鹅群中仅Hinf I酶切MC4R基因编码区存在RFLP,酶切得到HH和Hh 2种基因型,其1对等位基因H和h的频率分别为0.890 2和0.109 8。分析不同基因型与鹅胴体和羽绒性状之关联性表明,HH型与鹅屠体重、半(全)净膛重、肝脏重、心脏重、肌胃率、腹脂率、胸肌失水率以及绒比重具有显著的相关性,基因型效应对群体均数的贡献率在±5%之间;当MC4R基因突变后,Hh型的这种效应未能达到显著水平,但却与产脂力(3.314%)和脂肪酸品质(1.218%)(P<0.05)2个公共因子存在显著的相关性。
The carcass traits, meat quality traits and feather-down traits of 123 geese from F1 population of Wanxi White goose （male parent） and Sichuan White goose （female parent） were mensurated, and 12 common factors were extracted from the traits using factor analysis. The coding region of melanocortin receptor-4 （MC4R） gene was cloned, and the amplified products were digested with four restriction endonucleases Hinf Ⅰ,Ear Ⅰ,Pst Ⅰ and Pvu Ⅱ to detect restriction fragment length polymorphism （RFLP） in MC4R gene and its association with the traits. The results showed that the RFLP emerged only using Hinf Ⅰ with two genotypes HH and Hh, and frequencies of allele H and h were 0. 890 2 and 0. 109 8 in F1 population respectively. The effects of genotype HH were significant on carcass weight, eviscerated weight （with giblet）, liver weight, heart weight, stomach percentage, breast fat percentage, chest muscle water loss percentage and down proportion, and all genotypic contribution percentages （genotypic effect by population mean, CP） were between -5% and 5%, but on which the effects of genotype Hh were not significant after MC4R gene mutation. In effects of different genotype on 12 common factors, only the effects of genotype Hh were significant on fat power （CP = 3. 314%, P〈0.05） and fatty acid quality （CP = 1. 218%, P〈0.05）.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2008年第7期885-890,共6页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
安徽省十五人才计划基金(人才-4)
安徽省教育厅自然基金(2006KJ169B)资助
