摘要
目的建立兔高胆固醇-动脉粥样硬化模型,观察阿托伐他汀对脑组织的抗氧化应激保护作用。方法24只健康新西兰白兔随机分为3组:(A组)正常对照组、(B组)高脂饮食组和(C组)高脂饮食加阿托伐他汀组。喂饲8周后,测定兔血清总胆固醇(TC)及低密度脂蛋白(LDL)浓度,取脑组织检测血红素氧合酶(HO-1)水平、超氧化物歧化酶(SOD)活力及丙二醛(MDA)含量的变化。结果ABC三组HO-1平均吸光度值分别为0.418200±0.001874,0.682100±0.001853,0.918800±0.001549;SOD值分别为424.03±14.45、271.79±13.46和402.38±7.35;MDA值分别为16.66±2.55、40.87±2.78和21.57±2.19。与对照组相比,高脂饮食组HO-1表达显著上调,SOD活力下降,MDA含量增加(P<0.05);阿托伐他汀组较高脂饮食组HO-1表达进一步上调,SOD活力升高,MDA含量降低(P<0.05)。结论兔动脉粥样硬化过程中,脑组织处于氧化应激状态,阿托伐他汀可对抗氧化应激损伤,从而减缓动脉粥样硬化的形成。
Objective To establish model of rabbit with hypercholesterolemia-AS and investigate the influence of Atorvastatin to the expression of heine oxygenase-1 （HO-1）,SOD and MDA in the brain tissues. Methods Twenty-four healthy New-Zealand albino rabbits were divided into three groups randomly： （A group） normal control, （B group） high cholesterol diet, （C group） high cholesterol diet and Atorvastatin. After feeding for eight weeks, we would collect the empty stomach blood samples of these rabbits and determine the density of the total cholesterel（TC）and low density lipoprotein（LDL）. To extract their brains and detect the expression of HO-1, the activity of SOD,the amount of MDA. Results Average optical density of HO-1 in A,B and C group respectively： 0.418200 ±0.001874, 0.682100 ±0.001853, 0.918800 ±0.001549. The activity of SOD respectively：424.03 ± 14.45,271.79 ± 13.46,402.38 ±7.35. The amount of MDA respectively： 16.66 ±2.55,40.87 ±2.78,21.57 ±2.19. The HO-1 expression of high cholesterol diet group is increased significantly compared to normal control group. While the activity of SOD was lowered and the amount of MDA was increased （P〈0.05）. The HO-1 expression of Atorvastatin Group is increased significantly compared to High Cholesterol Diet Group .While the activity of SOD was increased and the amount of MDA was lowered （P〈0.05）. Conclusion The brain tissue is at the state of oxidative stress when AS taking place. Atorvastatin can alleviate this oxidative stress damage on brain tissue and step down the proceeding of AS.
出处
《中国心血管病研究》
CAS
2008年第7期545-547,共3页
Chinese Journal of Cardiovascular Research
关键词
高胆固醇
氧化应激
阿托伐他汀
Hypercholesterelemia
Oxidative stress
Atorvastatin
