摘要
为探讨Ｔｈ１与Ｔｈ２两类细胞因子间相互调节，研究了ｒｈＩＬ－１０对ＰＨＡ体外诱导人ＰＢＭＣ产生ＩＬ－２及ＩＦＮ－γ的影响。结果表明，１０ｎｇ／ｍｌＩＬ－１０明显抑制ＰＢＭＣ产生ＩＬ－２及ＩＦＮ－γ（Ｐ＜０．０２及Ｐ＜０．０１）。在加入兔抗人ＩＬ－１０抗体的ＰＢＭＣ体外培养体系中，在５μｇ／ｍｌ浓度时，ＩＬ－２产生可明显增加（Ｐ＜０．０５），随着抗体浓度增加，ＩＬ－２呈剂量依赖性增加（ｒ＝０．９６２，Ｐ＜０．０１），ＩＦＮ－γ产生亦增加，但差异不明显。本研究表明ＩＬ－１０抑制活化人ＰＢＭＣ体外产生ＩＬ－２及ＩＦＮ－γ，而抗人ＩＬ－１０抗体则表现出增强作用。ＩＬ－１０介导Ｔｈ１与Ｔｈ２两类辅助性Ｔ细胞间的相互调节，选择机体对抗原的免疫应答类型，从而对临床疾患的防治有一定指导意义。
In order to explore the regulation between Th1 and Th2 cytokines,the author studied the effect of recombinant human IL 10 on in vitro IL 2 and IFN γ production of PHA activated human peripheral blood mononuclear cells(PBMC).The results showed 10 ng/ml IL 10 inhibits IL 2 and IFN γ production by PBMC(P<0 02 and P<0 01,respectively).For concentration of 5 μg/ml rabbit anti human IL 10 antibody enhances IL 2 production(P<0 05),and in a dose dependent fashion.The antibody also increases IFN γ production,but there was no significant difference.The data confirm that the production of IL 2 and IFN γ by PHA activated PBMC was inhibited by IL 10 and enhanced by anti human IL 10 antibody.The regulation between Th1 and Th2 cells mediated by IL 10,choosing the types of immune response to different antigens are of certain guided significance for the prevention and treatment of clinical diseases.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1997年第5期317-319,共3页
Chinese Journal of Immunology
基金
深圳市科技计划
