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FTA卡在模板DNA制备中的应用研究 被引量：9

Research on application of FTA filters in preparation of DNA template
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摘要 目的:本文详细介绍了一种简便、快速、安全的用于DNA制备的方法。方法:查阅了大量国内外关于FTA卡及其应用方面的文章。结果:详细介绍了FTA卡在模板DNA制备以及在基因的储藏、医学检测以及食源性致病菌的检测方面的具体应用。结论:FTA卡可作为一种通用、快速、简便的DNA模板制备方法可用于生物学各方面的研究。 Objective：This article details a novel method that was used for DNA template preparation. Methods：In the present study, we consulted abundance of articles about FTA filter. Results：The use of FTA filter in gene storage, medicine, environment were specially studied. Conclusion： Using filters provides a faster and more sensitive and universal process for preparing DNA template.
作者 付素兰 刘景武 任天红 王国文
机构地区 石家庄市第三医院检验科
出处 《中国卫生检验杂志》 CAS 2008年第6期1124-1125,1150,共3页 Chinese Journal of Health Laboratory Technology
关键词 DNA模板制备 FTA卡 DNA template preparation FTA filters
分类号 Q523 [生物学—生物化学]
  • 相关文献

参考文献9

  • 1Carrie BO, Allen SK Filter paper for preserving insect and pathogen DNA samples. 2004isect.
  • 2AL - Soud WA. Capacity of nine thermostable DNA polymerases to mediate DNA amplication in the presence of PCR - Inhibiting [ J ]. Appl Environ Microbiol, 1998,3748 - 3753.
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  • 6Palmer AO, KA Lampel. Extraction - free, filter - based template preparation for rapid and sensitive PCR detection of pathogenic parasitic protozoa[J]. J Clin Microbiol,2000,38:2271 -2277.
  • 7Petra W, K Rickard. Rapid quantification of yersinia enterocolitica in pork samples by a novel sample preparation method, flotation, and prior to Real -Time PCR[ J]. J Clin Microbiol,2004,42:1042 - 1047.
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  • 9刘景武,张伟,何俊萍,周巍,袁耀武.FTA滤膜用于PCR检测肉中的金黄色葡萄球菌[J].生物工程学报,2005,21(6):1009-1013. 被引量：23

二级参考文献15

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  • 5Dana DP , Grace J , Ruth N et al. Rapid method for screening dried blood samples on filter paper for Human Immunodeficiency Virus Type 1 DNA. J Clin Microbiol ,1999 , 37 (2) : 350 - 353
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  • 8Petra W, Rickard K. Rapid quantification of Yersinia enterocolitica in pork samples by a novel sample preparation method, flotation,prior to Real-Time PCR. J Clin Microbiol, 2004, 42: 1042 - 1047
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共引文献22

同被引文献63

  • 1刘景武,张伟,何俊萍,周巍,袁耀武.FTA滤膜用于PCR检测肉中的金黄色葡萄球菌[J].生物工程学报,2005,21(6):1009-1013. 被引量：23
  • 2罗玉柱,成述儒,Batsuuri Lkhagva,D.Badamdorj,Olivier Hanotte,韩建林.用mtDNA D-环序列探讨蒙古和中国绵羊的起源及遗传多样性[J].Acta Genetica Sinica,2005,32(12):1256-1265. 被引量：38
  • 3张守发,于龙政,刘冰.FTA纸片中保存的马巴贝虫DNA的PCR检测[J].中国兽医科学,2006,36(8):647-649. 被引量：5
  • 4Shao JW,Wu ZX,Guo YH,et al.Booster-PCR amplification and sequencing of gene encoding β-sub-unit of glycerol dehydratase.Journal of Food Science and Biotechnology,2005,24(5):86-88.
  • 5Zhou H,Hickford JGH,Fang Q.A two-step procedure for extracting genomic DNA from dried blood spots on filter paper for polymerase chain reaction amplification.Analytical Biochemistry,2006,354(1):159-161.
  • 6Lois CT,Michelle T,Reich K.Automated Forensic DNA Purification optimized for FTA card punches and identifiler STR-based PCR analysis.Journal of the Association for Laboratory Automation,2005,10(4):231-236.
  • 7Vanek D,Hradil R,Budowle B.Czech population data on 10 short tandem repeat loci of SGM plus STR system kit using DNA purified in FTA cards.J Forensic Science International,2001,119(1):107-108.
  • 8Krenke BE,Tereba A,Anderson SJ,et al.Validation of a 16-locus fluorescent multiplex system.J Forensic Sci,2002,47 (4):73-785.
  • 9Panteleefe DD,Nduati RJG,et al.Rapid method for screening dried blood samples on filter paper for human immunodeficiency virus type 1 DNA.J Clin Microbiol,1999,37(2):350-353.
  • 10Ingrid AB,Kathryn DD,Ann JM,et al.Simple,sensitive,and specific detection of human immunodeficiency virus type 1 subtype b DNA in dried blood samples for diagnosis in infants in the field.Journal of Clinical Microbiology,2001,39 (1):29-33.

引证文献9

二级引证文献17

中国卫生检验杂志
2008年 第6期

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