摘要
用 1μmol/ L甲基紫精对玫瑰茄培养细胞进行 16h和 2 4 h暗诱导 ,其 SOD活力分别比对照增大 74 .87%和 32 4 .2 4 % ,比活力分别增加 69.99%和 2 95.73%。经 2 4 h暗诱导的细胞 ,在1mmol/ L甲基紫精的作用下 ,其电解质渗漏率比对照下降了 4 8% ,且差异达到极显著水平 (P≤0 .0 1)。这表明细胞中 SOD含量与电解质渗漏率密切相关 ,SOD含量高者 ,其电解质渗漏率低。以此为依据 ,建立了一种评价高产 SOD植物细胞的体外模型 ,即以 lmmol/ L 甲基紫精作为评定阈值 ,直接根据电解质渗漏率的变化来衡量细胞中 SOD含量的高低 ,为筛选高产 SOD细胞提供了简便有效的方法。利用这一模型 ,评价了中草药、环境污染物质和巯基化合物在提高
The enzyme superoxide dismutase (SOD)activity and specific activity of cultured roselle (Hibiscus sabdariffa)cells,induced by 1μ mol/L methylviologen for 16 hours and 24 hours in the dark,were increased respectively by 74 87%,324 24% and 69 99%,295 73% when compared with the control At a concentration of 1mmol/L of methylviologen, the electrolyte leakage of cells induced for 24 hours was decreased by 48% as compared with the control, and the difference was very significant ( P ≤0 01) This result shown that the content of SOD in cells was closely related with the electrolyte leakage:cells with higer content of SOD had lower percentage of electrolyte leakage Based on the conclusion,an in vitro model for evaluating SOD overproduction plant cells was constructed:a concentration of 1mmol/L methyl viologen was chosen as a threshold value for evaluation and the level of SOD in cells was measured simply based on the change of the electrolyte leakage percentage It provided an simple and effective means of screening for SOD overproduction cells This model was used to evaluate the role of some Chinese herb medicine, environmental contaminants and sulfhydryl compounds on elevating SOD production
出处
《药物生物技术》
CAS
CSCD
1997年第3期144-149,共6页
Pharmaceutical Biotechnology
基金
国家自然科学基金资助项目
关键词
药物
玫瑰茄细胞
SOD
生物膜
评估模型
Roselle cells,SOD activity,Biomembrane,Free radicl,Induction
