摘要
近年来有观点认为溃疡性结肠炎（ulcerative colitis，uc）是一种神经源性炎症。我们实验室曾报道，以脊髓蛛网膜下腔（intrathecal，ith）注射半抗原二硝基氯苯（2，4-dinitrochlorobenzene，DNCB）的方法，在致敏大鼠建立了结肠炎模型。本研究拟进一步探讨此结肠炎过程中神经免疫介导物移动抑制因子（migration inhibitory factor，MIF）是否参与其发病机制。选用7-9周龄健康雄性Sprague-Dawley大鼠，用免疫荧光双染法分别测定ith注射DNCB后肠壁经组织和脊髓组织MIF蛋白的表达。观察MIF抗体预处理对ith注射DNCB后大鼠的疾病活动指数（disease active index，DAI）评分和结肠组织病理变化的影响。结果表明：ith注射DNCB大鼠的结肠神经组织和脊髓组织MIF蛋白的荧光强度显著高于ith注射乙醇（对照）组；MIF抗体（1：10，1：5）预处理能够显著减轻由ith注射DNCB引起的DAI高评分和结肠病理变化。上述结果提示，肠和脊髓神经组织MIF活性升高或，和释放增多是im注射DNCB后结肠炎发生的一个重要原因，神经免疫机制参与了ith注射DNCB引起的大鼠结肠炎过程。
In recent years, there has emerged academic tendency towards the neurogenic mechanism of ulcerative colitis （UC）. As one of the supports to the hypothesis of UC being a neurogenic inflammation, we have built a colitis model by intrathecal （ith） injection of a haptten 2,4-dinitrochlorobenzene （DNCB） to DNCB-sensitized rats. In order to explore further the neuroimmunal mechanism of this colitis model, we here focused on a pro-inflammatory cytokine, migration inhibitory factor （MIF）, to observe its expression in rat colon nervous tissue and spinal cord in the colitis induced by ith injection of DNCB. At the same time we also observed the effect of MIF antibody pretreatment on the disease active index （DAI） score and the colon pathology by HE staining in the colitis rats. The results Obtained showed that the immunofluorescence intensity of double staining of MIF protein in colon nervous tissue and spinal cord was increased in 0.8% and 1.6% DNCB-induced colitis groups than that in the control （60% ethanol） group. Both the colon pathology and the DAI score were significantly reduced by MIF antibody ith pretreatment. Ith injection of 0.8% DNCB after MIF antibody （1：10, 1：5） pretreatment could only induce lower DAI score （P〈0.01 as compared, respectively, to the IgG pretreatment group）. The colon pathological changes in ith 0.8% DNCB rats were mild, even little after MIF antibody （1：10, 1：5） pretreatment. These results suggest that MIF in spinal cord and enteric nervous system is possibly involved in the rat colitis induced by ith injection of DNCB, which reflects a neuroimmunal mechanism underlying this kind of colitis. MIF is possibly one of the important neurochemical factors in this experimental colitis, even in the UC.
出处
《生理学报》
CAS
CSCD
北大核心
2008年第3期419-424,共6页
Acta Physiologica Sinica
基金
Beijing Natural Science Foundation (No. 7063083)
the National Natural Science Foundation ofChina (No. 30170419)
