摘要
副溶血性弧菌是细菌性腹泻的主要致病因子．本研究建立一种快速标本处理和聚合酶链反应（PCR）诊断方法，扩增副溶血性弧菌的热稳定直接溶血素（TDH）基因。对一组56例腹泻患者粪便标本进行了检测，经细菌培养证实为副溶血性弧菌的9份标本，PCR法均阳性；培养法明性的39例中，有7例检测到TDH基因．统计学表明两种方法检测结果相当一致，但检出能力PCR法显著高于培养法。本法简便、快速、特异，适宜临床应用．
Vibrio Parahaemolyticus is the principal pathogenic factor causing acute bacterial diarrhea. A method using the rapid PCR to detect the virulence gene, the thermostable direct hemolysin (TDH)of Vibrio para-haemolyticus, in diarrheal stools was set up. In 56 cases of diarrhea, 9 V. parahaemolyticus isolated by con-ventional culture method were positive with PCR; and TDH genes of 7 in 39 culture-negative samples were identified by PCR in feces. Statistical P values of both correlation and difference were less than 0.01 by Chisquare test. The results f the two methods are identical and PCR was more sensitive than cnventinalmethod. It is shown that the novel method is rapid, sensitive and specific for diagnosis of V. parahaemolyt-icus, and suitable for clinical use.
出处
《上海医学检验杂志》
1997年第4期193-195,共3页
Shanghai Journal of Medical Laboratory Sciences
基金
全军八五科技攻关项目
