摘要
目的探讨寿胎丸对TA97、TA98、TA100、TA102菌株基因回变菌落的影响,对其遗传安全性进行研究。方法采用直接平皿掺入技术,设寿胎丸不同剂量组,并对TA97、TA98、TA100、TA102系列菌株进行体外染毒,观察其回变菌落增长状况。结果在0.8至200.0 mg/皿的不同测试浓度,+S9与-S9两种测试系统条件下,其作用的4种测试菌株的回变菌落数与阴性对照相比无明显增加,回变菌落背景正常,未显示其有抑菌作用,且无剂量-反应关系。结论寿胎丸药液在本试验所确定的200.0 mg/皿剂量范围内,初步认为对TA系列测试菌株无明显致DNA碱基置换及移码突变性。
Objective To investigate the effect of Shoutai pills on bacterial strains TA97,TA98, TA100 and TA102 of genie mutation bacterial strains TA97, TA98, TA100 and with different doses of Shoutai pills groups, Results The TA bacterial reverse mutation and study hereditary toxicity TA102 were poisoned with of them. Methods The utensil means in vitro and the DNA variety of TA bacterial was observed. induced by Shoutai pills was not increased under mixing S9 or non-mixing S9, compared with that in control group. Conclusions The Shoutai pills in the range of 200 mg/a utensil were not genotoxicity to TA bacterial aberration.
出处
《湖南中医药大学学报》
CAS
2008年第3期5-7,共3页
Journal of Hunan University of Chinese Medicine
基金
国家教育部高校博士点资助项目(20070541001)
湖南省博士后重点项目(2007RS4109)
关键词
寿胎丸
遗传毒性
AMES试验
安全性
菟丝子
续断
阿胶
Shoutai pills
genotoxicity
security
Ames bacterial reverse mutation test
Semen Cuscutae
teasel root
colla corii asini
