摘要
【目的】观察安心颗粒对慢性心力衰竭(CHF)模型大鼠血清肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)水平及心肌细胞凋亡的影响。【方法】将50只雌性SD大鼠随机分为正常对照组、模型组、卡托普利组、安心颗粒低剂量组和安心颗粒高剂量组。采用阿霉素腹腔注射造模,每周1次,连续6周。造模同时,卡托普利组及安心颗粒低、高剂量组分别按6.25mg.kg-1.d-1、0.8g.kg-1.d-1、1.6g.kg-1.d-1剂量灌胃给药。造模结束后,分别测定各组血清TNF-α、IL-6水平与心肌细胞凋亡指数(I凋亡)。【结果】模型组血清TNF-α、IL-6水平及I凋亡均显著性升高(P<0.05);安心颗粒低、高剂量组均可显著性降低模型大鼠血清TNF-α、IL-6水平及I凋亡(P<0.05或P<0.01),且呈现一定剂量效应关系。【结论】安心颗粒可通过调控炎性细胞因子,抑制心肌细胞凋亡,阻止心力衰竭的发展。
Objective To establish the relationship of serum TNF-α and IL-6 contents with myocardial cell apoptosis in the chronic heart failure （CHF） rats, and to explore the therapeutic mechanism of Anxin Granules （AXG） in treating CHF. Methods Fifty female SD rats were randomly divided into five groups： normal control group （A） , model group （ B）, captopril group （C） , low-dosage AXG group （D） and high-dosage AXG group （E）. CHF models were established by intraperitoneal injection of small-dose of adriamycin, once per week for continuous 6 weeks. Meanwhile, group C was given captopril injection （6.25 mg ·kg^-1·d^-1 ）, and groups D and E received gastric gavage of low-dosage AXG （0. 8 g ·kg^-1·d^-1） and high-dosage AXG （ 1.6 g ·kg^-1·d^-1 ） respectively. After 6 weeks of modeling, serum levels of TNF-α and IL-6 of different groups were tested by ELISA, the apoptosis of myocardial cells was tested by TUNEL, and then the apoptosis index （AI） was counted. Results Serum levels of TNF-α and IL-6 were increased in the model group （P 〈 0. 05 compared with group A）. Low- and high-dosage AXG decreased the serum levels of TNF-α and IL-6 as well as AI of the model rats （P 〈 0. 05 or P 〈 0. 01 ）, in a dose-effect manner. Conclusion AXG can inhibit the apoptosis of myocardial cells by regulating the inflammatory factors, thus restrain the development of CHF.
出处
《广州中医药大学学报》
CAS
2008年第4期307-310,共4页
Journal of Guangzhou University of Traditional Chinese Medicine
基金
广西自然科学基金项目(编号:0728173)
