摘要
目的:了解北京地区儿童急性上呼吸道感染病毒病原学现况,探讨不同年龄组、不同月份病毒检出情况,为预防策略制定、临床诊断、治疗提供病原学依据。方法:采用多重RT-PCR法,对365份咽拭子样本同时检测腺病毒,偏肺病毒,冠状病毒229E,1、2、3型副流感病毒;甲、乙型流感病毒,甲、乙型呼吸道合胞病毒,甲型鼻病毒,冠状病毒OC43共12种常见呼吸道病毒。结果:365份样本中有阳性检出结果的有246份,阳性率为63.08%,其中有2种以上病毒混合感染65份,占17.80%;6个月以下患儿以甲型呼吸道合胞病毒检出阳性率最高;6个月以上至14岁患儿中以甲型流感病毒检出阳性率最高;2006年10月及2007年4月腺病毒检出率最高,11月份与2月份以呼吸道合胞病毒检出率最高,12月与1月份以流感病毒检出率最高;在阳性检出样本中流感病毒占52.85%、呼吸道合胞病毒占26.42%、腺病毒占18.70%。结论:流感病毒、呼吸道合胞病毒、腺病毒为北京地区儿童急性上呼吸道病毒感染主要致病原,随年龄不同、流行季节中月份不同而有一定的流行规律。
Objective：To study the situation of the virus etiology in children with acute upper respiratory infection in Beijing and to discuss the respiratory viruses epidemiology for different age groups in different months so as to provide the data for the preventive strategy, clinical diagnosis and treatment. Methods ： By multiplex RT - PCR, adenovirus, metapneumovirus, coronavirus229E, parainfluenza virus 1,2,3, influenza virus A, B, respiratory syncytial virus A, B, rhinovirus A and coronavirus OCA3 12 kinds of common respiratory viruses totally were detected in 365 clinical swabs simultaneously. Results ： 246 positive results were got from the 365 samples, the positive ratio was 63.08%. The coinfection caused by more than 2 kinds of viruses were 65 whose proportion was 17. 8%. For the under- 6 months children, the respiratory syncytial virus was maximum, for the children between 6 months and 14 years influenza A virus was found largest. In Oct. of 2006 and Apr. of 2007, the adenovirus was the largest while in Nov. of 2006 and in Feb. of 2007, the respiratory syncytial virus was the most detected. In Dec. of 2006 and Jan. of 2007 the influenza A virus was the main pathogen. In all the positive results the influenza virus was 52. 85% , respiratory syncytial virus was 26. 42% and the adenovirus was 18.70%. Conclusion： Influenza virus, respiratory syncytial virus, adenovirus were the main pathogens leading to the children acute upper respiratory infection in Beijing and the pathogens have specific epidemiology according to the ages and the seasons.
出处
《中国卫生检验杂志》
CAS
2008年第7期1263-1265,共3页
Chinese Journal of Health Laboratory Technology
