摘要
目的:采用高效液相色谱法测定祖卡木胶囊中大黄素和大黄酚的含量。方法:采用Waters Symmetry C_(18)(150mm×4.6mm,5μm)色谱柱,流动相:甲醇-0.1%磷酸溶液(80:20);检测波长:254 nm;流速1.0 ml·min^(-1);柱温35℃。结果:大黄素在0.8～6.6μg·ml^(-1)(r=0.999 9,n=5)范围内,有良好的线性关系,平均回收率为98.8%(n=9),RSD为1.7%。大黄酚在2.0～10.2μg·ml^(-1)(r=0.999 9,n=5)范围内,有良好的线性关系,平均回收率为101.3%(n=9),RSD为1.6%。结论:方法具有操作简单、准确的优点,能有效控制祖卡木胶囊的质量。
Objective： To establish an HPLC method for determination the contents of demodin and chrysophanol in zukam capsules. Method： The Water Symmetry C18 （ 150 mm ×4.6 mm,5 μm） was used, the mobile plase consisted of methanol -0.1% phos- phoric acid solution（80： 20）, the flow rate was 1.0 ml·min^-1, the detection wavelength was 254 nm, the column temperature was 35 ℃. Result： The linear ranges of emodin and Chrysophanol were at 0.8 -6.6 μg·ml^-1 （r=0. 999 9） and 2.0 - 10.2 μg·ml^-1 （r =0.999 9）, the average recoveries（n =9） were 98.8% and 101.3% with RSD 1.7% and 1.6% respectively. Conclusion： The method is simple and accurate, it can be used for quality control of zukam capsules.
出处
《中国药师》
CAS
2008年第7期772-774,共3页
China Pharmacist
