摘要
目的研究脂多糖对诱导分化的牙髓细胞牙本质涎磷蛋白（dentin sialophosphoprolein，DSPP）、骨钙素基因表达及碱性磷酸酶（alkaline phosphatase，ALP）活性的影响。方法以脂多糖作用于诱导分化的牙髓细胞，用荧光定量反转录聚合酶链反应（RT-PCR）检测DSPP、骨钙素mRNA的表达，ALP试剂盒检测ALP活性改变。结果诱导分化的牙髓细胞在脂多糖刺激后ALP活性由（1156，10±100，60）pmol·h^-1·ng^-1下降为（884．80±26，72）pmol·h^-1·ng^-1，荧光定量RT-PCR显示脂多糖能显著抑制DSPP和骨钙素mRNA表达，其中DSPP拷贝数由（176301．62±13269．77）下降为（39396，32±12018．08），骨钙素拷贝数由（4971．46±483，91）下降为（1104.67±63．37）（t=7．922，P〈0．001）。结论脂多糖能显著降低诱导分化的牙髓细胞合成有机基质。
Objective To investigated the effect of Escheerichia coli （Ec） LPS on alkaline phosphatase（ALP） activity and expression of dentin sialophosphoprotein （DSPP） and osteocalcin （OCN） genes in vitro differentiation human dental pulp cell. Methods Odontoblast-llke ceils were cultureed, cells exposed to Ec LPS for 12 h ,total RNA was isolated and DSPP,OCN transcripts were examined by real-time RT-PCR. ALP kit were used to assessed the changes of ALP activity. Results Real-time RT-PCR analysis indicated that Ec LPS induced about a 3. 6-fold decrease for DSPP gene and a 1.6-fold decrease for OCN gene in odontoblast-like cells as compared with controls. At the same time, cells treated with LPS could depress ALP activity from （ 1156. 10 ± 100. 60） ）pmol·h^-1·ng^-1 down to （ 884.80± 26, 72 ））pmol·h^-1·ng^-1, Conclusions These results indicate that exposure of odontoblast-like cells to LPS can alter cells function by downregulateing cell markers of odontoblastic activity.
出处
《中华口腔医学杂志》
CAS
CSCD
北大核心
2008年第7期429-430,共2页
Chinese Journal of Stomatology
关键词
牙髓
脂多糖类
矿化基质
Dental pulp
Lipopolysacearides
Mineralized matrix
