摘要
从天麻中提取分离天麻多糖(GBP-Ⅰ,GBP-Ⅱ),并对其组成进行研究。天麻依次经热水浸提,乙醇沉淀,Sevag法除蛋白质,有机溶剂洗涤、超滤,得天麻多糖GBP-Ⅰ和GBP-Ⅱ,将GBP-Ⅰ和GBP-Ⅱ分别用1mol/L的盐酸在100℃水解,水解液经过离子色谱分析,确定其单糖组成的种类和比例。GBP-Ⅰ和GBP-Ⅱ两种多糖均由鼠李糖、半乳糖、葡萄糖、木糖、甘露糖组成。其中GBP-I中5种单糖的相对含量分别是1.27%、5.41%、76.49%、6.32%、10.51%,GBP-Ⅱ中5种单糖的相对含量分别是2.32%、8.49%、62.44%、10.22%、16.53%。
Extraction and separation of Polysaccharide from Gastrodia elata Blume （GBP- Ⅰ , GBP- Ⅱ ）, study the composition of polysaccharides.The polysaccharides were extracted from Gastrodia elata Blume by hot water,respectively treated with ethanol precipitation and Sevag method,washing with organic solvent,ultrafihration,then GBP- Ⅰ and GBP-Ⅱ were obtained.GBP- Ⅰ and GBP-Ⅱ were hydrolyzed with 1mol/L hdrochloric acid at 100℃,and ion chromatography was used to determinate the composition of monosaccharide of hydrolyzate.GBP-Ⅰ and GBP-Ⅱ were both composed of rhamnose, galactose,glucose, xylose and mannose the relative contents of rhamnose, galactose, glucose, xylose and mannose of GBP-Ⅰ were 1.27%, 5.41%,76.49%,6.32%,10.51%,and five monosaccharide of GBP-Ⅱ were respectively 2.32%,8.49%,62.44%, 10.22%,16.53%.
出处
《中国农学通报》
CSCD
2008年第7期89-92,共4页
Chinese Agricultural Science Bulletin
基金
2005年西北农林科技大学青年学术骨干支持计划
2005年度教育部新世纪优秀人才支持计划(NCET-05-0855)
兰州大学应用有机化学国家重点实验室开放基金
关键词
天麻多糖
超滤
离子色谱
单糖
Gastrodia elata blume polysaccharides, ultrafiltration, ion chromatography, monosaccharide
