摘要
乳酸链球菌素(Nisin)是由乳酸链球菌产生的含有34个氨基酸的抗菌肽,对人体无害。本研究将患临床型乳房炎奶牛92头(107个乳区)和隐性乳房炎奶牛90头(90个乳区)随机分成试验组和对照组。试验组的治疗方法是在患病乳区灌注Nisin;临床型乳房炎以庆大霉素乳房内灌注作对照,隐性乳房炎的对照组不作任何处理。临床型乳房炎的Nisin治疗组细菌学治愈率和临床治愈率分别为60.8%和90.2%,而庆大霉素治疗组则分别为44.6%和91.2%,两者无统计学差异。隐性乳房炎经Nisin治疗后,高体细胞数奶牛的数量和牛奶中NAGase的活性均显著下降,细菌转阴率提高。乳酸菌发酵试验表明,经Nisin乳房内灌注治疗临床型乳房炎后,牛奶在第36小时恢复正常发酵,而庆大霉素治疗则需要72h。
Nisin is an antibacterial peptide of 34 amino acids produced by Lactococcus lactis, and is harmfulness for human. In this study, 92 cows with 107 clinically mastitis quarters and 90 cows with 90 recessive mastitic quarters were randomly divided into nisin-treated and control groups. In nisin-treated groups, intramammary infusion of a nisin-based preparation was administered. In the control groups, intramammary infusion of gentamycin was used for clinical mastitis and no treatment was used for recessive mastitis. The results indicated that bacteriological and clinical cure rates were 60.8% and 90.2% in nisin-treated clinical mastitis cases respectively, while 44.6% and 91.2% in gentamycin-treated group respectively. There was no statistical difference between two groups. After nisin treatment in recessive mastitis cases, cows with high milk somatic cell count and milk NAGase activity were significantly decreased, and milk samples negative to bacteriological examination were increased. Inhibitory effect on fermentation was found until 36 hours after nisin treatment and 72 hours after gentamycin treatment.
出处
《中国奶牛》
2008年第7期41-44,共4页
China Dairy Cattle
基金
浙江省重大科技项目(2005C1205)
杭州市重大科技项目(20052112A06)
