摘要
目的 制备新糖蛋白opticin特异性抗体并探讨opticin在人眼中的分布。方法 先合成一段多肽链（CLPRLPIGRFT），将该多肽链免疫兔以获得opticin抗体，通过对人玻璃体提取物进行免疫印迹检测以验证该抗体的有效性，用免疫组织化学方法检测opticin在人眼中的分布。结果 人眼玻璃体提取物免疫印迹检测，在相对分子质量45 ×10^3～50×10^3处可看到一个宽范围的条带。人眼免疫组织化学发现opticin存在于人眼视网膜、玻璃体和睫状体无色素上皮中。在玻璃体内opticin沿着胶原纤维而分布。结论 人眼玻璃体提取物免疫印迹检测证实了opticin抗体的有效性。opticin主要存在于视网膜、睫状体无色素上皮及玻璃体中。沿着胶原纤维分布的opticin可能与玻璃体结构的稳定有关。
Objective To prepare a new glycoproteinopticin specific antibody and to explore the distribution of opticin in human eye. Methods Firstly, take the opticin specific antibody to compound a synthetic peptide chain（CLPRLPIGRFT）, and then get the opticin antibody. To verify the availability of antibody through the western blot for human vitreous extract, to test the distribution of opticin in human eye by immunohistochemistry. Results Through the western blot for human vitreous extract, we can see a band with wild range at molecular weight 45×10^3～50 ×10^3. We find that opticin exact in retina, vitreous and non-pigmented epithelium of ciliary body which distributes along the collagen fibrils in vitreous. Conclusion The availability of the antibody was confirmed by western blot. Opticin are mainly in retina, vitreous and nonpigmented epithelium of ciliary body. Opticin distributes along the collagen fibrils which may be related to the stability of vitreous.
出处
《中华眼底病杂志》
CAS
CSCD
北大核心
2008年第4期286-288,共3页
Chinese Journal of Ocular Fundus Diseases
基金
武汉市晨光计划资助项目（200750731255）.志谢 感谢广州军区武汉总医院眼科金中秋主任、周和政主任、晏颖医师和武汉大学人民医院眼科艾明、杨燕宁老师在提供实验材料方面给予的帮助
感谢武汉大学实验动物中心唐志佼、王勇、包容老师,武汉大学基础医学院遗传学教研室杨勇老师在免疫组织化学实验中给予的帮助.
关键词
糖蛋白类/生理学
组织分布
玻璃体/生理学
Glycoproteins/ physiology；Tissue distribution；Vitreous body/ physiology
