摘要
从150条RAPD随机引物中筛选出多态性引物12条,对分离自福建省10个不同市(县)的番茄或马铃薯晚疫病标样中的63个致病疫霉菌株进行遗传多样性分析,共产生92条RAPD条带,其中85条为多态性条带,多态检测率为92.4%。利用NTSYSpc Version 2.1软件对供试菌株间的遗传距离进行聚类分析并构建系统树状图,供试63个菌株被划分为6个遗传聚类组,RAPD分组与菌株的地理来源、寄主均无明显相关性。聚类分析结果表明,福建省不同地区的致病疫霉菌株整体亲缘关系相近,但各菌株间存在遗传差异,病原菌随病果运输迁移及A2交配型的存在可能是导致这种现象的原因。
Genomic fingerprints of 63 strains of the pathogen, Phytophthora infestans, from different areas and hosts were analyzed by using 12 RAPD primers. These primers were randomly selected from 150 primers with abundant polymorphisms. Genomic DNA from the different strains were amplified with the 12 selected primers resulting in 92 marked bands. Among the bands, 85 were polymorphic, which accounted for 92.4% of the total bands produced. Based on the genetic distance, a dendrogram was constructed with the software NTSYSpc Version 2.1. Of the 63 strains tested, 6 genetic groups were formed. There was no correlation found between RAPD group and the geographic origin or their host. The analysis showed that some strains from one area were closely related to some from another area. Nonetheless, rich genetic variation existed among the tested strains. Migration and A2-type mating of the late blight fungus might have been the cause of the diversity.
出处
《福建农业学报》
2008年第2期149-153,共5页
Fujian Journal of Agricultural Sciences
基金
国家自然科学基金项目(30600401)
福建省自然科学基金项目(2006J0322)
福建省财政专项(STIF-Y07)
农业部公益性行业(农业)科研专项(nyhyzx07-053)
关键词
致病疫霉
晚疫病
RAPD
遗传多样性
Phytophthora infestans
late blight RAPD genetic diversity
