摘要
在小麦背景中离果山羊草3C染色体具有优先传递的作用。当离果山羊草3C染色体处于单体状态时会导致后代不含有杀配子染色体的配子中产生包括缺失和易位等染色体结构变异。簇毛麦4V染色体携有抗小麦眼斑病和全蚀病基因。为进一步利用簇毛麦4V染色体上的有益基因,利用染色体C-分带和基因组原位杂交分析,从普通小麦-簇毛麦4V染色体二体异附加系(DA4V)与普通小麦农林26-离果山羊草3C染色体二体异附加系(DA3C)杂种后代中选育出小麦-簇毛麦纯合易位系T4VS·6AL。该易位系为杀配子染色体诱发形成的非补偿型易位;易位系T4VS·6AL高抗梭条花叶病,是小麦抗病育种的新种质。
Chromosome 3C of Aegilops triuncicdis were of the ability to be transferred preferentially in the case of its monosomic status in wheat background. Whereas, those gametes without 3C would result in chromosome structural changes including deletion and translocation. Eyespot and take-all resistance gene was mapped on chromosome 4V. The objective of this study was to develop more translocation lines involving chromosome 4V, and provided new germplasm for wheat breeding. Triticum aestivum-Haynaldia villosa 4V disomic addition line was crossed with T. aestivum ＂Norin 26＂-Ae. triuncicdis 3C disomic addition line , and the hybrids F1 were then self pollinated. Both chromosome C-banding and fluorescent in situ hybridization （FISH） were applied to screen chromosome variations of 4V. One heterozygous translocation llne involved in 4V and 4A （CQZ98） was identified from F2, and a homozygous translocation line （CQZ98-1） was selected from the offspring of CQZ98 and confirmed by chromosome pairing analysis at meiosis Ⅰ of pollen mother ceils （PMC）. The results of simple sequence repeat（SSR）analysisindicated that CQZ98-1 did not contain 4VL, and designated as T4VS·6AL. This translocation line showed high resistance to wheat spindle streak mosaic virus.
出处
《湖北农业科学》
北大核心
2008年第6期611-614,共4页
Hubei Agricultural Sciences
基金
国家自然科学基金项目(30200175)
江苏省教育厅自然科学基金(05KJD180125)
南京晓庄学院重点课题(2005NXY01)
关键词
普通小麦
簇毛麦
T4VS·6AL易住
C-分带
荧光原位杂交
Triticum aestivum
Haynaldia villosa
T4VS·6AL translocation
C-banding
fluorescent in situ hybridization
