摘要
目的探讨5-氨基乙酰丙酸(5-ALA)光动力学疗法(photodynamic therapy,PDT)对体外培养人结肠癌LoVo和CoLo205细胞的生物作用。方法于体外培养的LoVo和CoLo205细胞分别加入不同浓度的5-ALA(0.1、0.5、1.0、1.5、2.0和2.5 mmol/L)孵育6 h,以波长为630 nm的半导体激光进行照射,照射的功率密度为20mW/cm^2,照射能量密度分别为2、5、10、20 J/cm^2,继续孵育24h,用四唑盐(MTT)比色法测定细胞存活率;荧光分光光度计检测LoVo与CoLo205细胞内PpⅨ荧光强度。结果5-ALA-PDT对体外培养的人结肠癌LoVo和CoLo205细胞均有杀伤作用,两者无显著性差异(P>0.05)。其杀伤作用大小与5-ALA的浓度和激光剂量成正相关(P<0.01)。激光能量为20 J/cm^2,5-ALA的浓度为1.0 mmol/L时,其杀伤LoVo、CoLo205细胞的作用最明显;LoVo与CoLo205细胞内PpⅨ荧光强度差异无显著性意义(P>0.05)。结论5-ALA-PDT可有效杀伤体外培养的人结肠癌LoVo和CoLo205细胞,且对两种细胞的杀伤作用无显著性差异。
Objective To investigate the potential use of 5-aminolevulinic acid （5-ALA）-photodynamic therapy （PDT） of colon carcinoma cell lines LoVo and CoLo205 cultured in vitro. Methods LoVo and CoLo205 cells cultured in vitro were incubated in a medium contained various concentrations of 5-ALA （0.1,0.5, 1.0,1.5,2. 0 or 2. 5 mmol/L） for 4 hours and illuminated with different light doses （2,5,10 or 20 J/cm^2 ） using a semiconductor laser at 630 nm. After 24 hour incubation with 5-ALA-PDT, the survival rate of LoVo and CoLo205 cells was analyzed by MTT assay. The cellular fluorescence intensities of PpⅨ in LoVo and CoLo205 were measured with a luminescence spectrometer. Results 5-ALA-PDT may effectively kill the LoVo and CoLo205 cells cultured in vitro,while the killing effect showed no significant difference between them（ P 〉0.05 ）. The killing degrees were positively correlated with the concentration of 5-ALA and the dosage of radiation. The cellular PpⅨ fluorescence intensities had no significant difference between the two kinds of cells. Conclusions 5-ALA-PDT may effectively kill the LoVo and CoLo205 cells cultured in vitro.
出处
《中国激光医学杂志》
CAS
CSCD
2008年第3期162-165,共4页
Chinese Journal of Laser Medicine & Surgery
基金
中国临床肿瘤学科学基金(Y20050013)
关键词
结肠癌
5-氨基乙酰丙酸
光动力学疗法
Colon carcinoma
5-Aminolevulinic acid （5-ALA）
Photodynamic therapy （PDT）
