摘要
目的观察慢性氟中毒大鼠脑组织及血浆氧化应激水平的改变，探讨氟中毒性神经损害发病机制中氧化应激水平与学习记忆的关系。方法SD大鼠按体质量随机分为3组，每组8只。对照组：自由饮用自来水，自来水含氟量低于0．5mg／L；低剂量染氟组：饮水含氟量为5mg／L；高剂量染氟组：饮水含氟量为50mg／L。实验期为6个月，检测大鼠学习记忆功能、血浆和脑组织总抗氧化能力（T—AOC）及丙二醛（MDA）水平。结果高剂量染氟组大鼠逃避潜伏期[（14．37±3．48）s]长于对照组[（5．84±1．87）s]和低剂量染氟组[（7．18±1．42）s]，两组间比较差异有统计学意义（P〈0．05）；高剂量染氟组、低剂量染氟组大鼠血浆和脑组织T—AOC水平[（1．37±0．27）×10^3U／L、（0．24±0．06）×10^3U／g Pr，（1．20±0．14）×10^3U／L、（0．41±0．10）×10^3U／g Pr]低于对照组[（2．17±0．11）×10^3U／L，（0．79±0．11）×10^3U／g Pr]，两组间比较差异有统计学意义（P〈0．05）；高剂量染氟组大鼠血浆和脑组织MDA水平[（3．72±0．59）mmol／L、（4．01±0．21）mmol／g Pr]显著高于对照组[（2．34±0．16）mmol／L、（2．97±0．11）mmol／g Pr]和低剂量染氟组[（2．68±0．33）mmol／L、（3．38±0．21）mmol／g Pr]，两组间比较差异有统计学意义（P〈0．05）。结论慢性氟中毒可使机体氧化应激水平升高，大鼠学习记忆能力减退可能与氧化应激水平的升高有关。
Objective To investigate the changes of oxidative stress level in brain tissues and serum, and learning and memory in rats with oxidative stress level in nerve damage in chronic fluorosis. Methods The rats were randomly divided into 3 groups according to the body weight, eight rats in each group, i.e., control group, drinking water containing less than 0.5 mg/L of fluoride; lower fluoride exposure group, drinking water containing 5 mg/L of fluoride; higher fluoride exposure group, drinking water containing 50 mg/L of fluoride. The animals were examined six months after initiating the experiment. The total antioxidant capacity （T-AOC） and malondialdehyde （MDA）, as well as learning and memory, were measured. Results Escape latency in higher fluoride exposed group[ （14.37 ± 3.48）s] was significantly higher than that of controls[ （5.84 ± 1.87）s] and exposed to lower fluoride [ （7.18 ± 1.42）s], the difference being statistically significant（P 〈 0.05 ）. As compared with controls[ （2.17 ± 0.11 ） ×10^3U/L , （0.79 ± 0.11 ） ×10^3U/g Pr] ,the rats exposed to higher fluoride and lower fluoride exhibited lower levels of T-AOC [（1.37 ± 0.27） ×10^3 U/L,（0.24 ± 0.06） ×10^3 U/g Pr and （1.20 ± 0.14） ×10^3 U/L, （0.41 ± 0.10） ×10^3 U/g Pr], the difference being statistically significant（P 〈 0.05）. As compared with controls[ （2.34 ± 0.16） mmol/L, （2.97 ± 0.11 ）mmol/g Pr] and low fluoride exposed group[ （2.68 ± 0.33 ）mmol/L, （3,38 ± 0.21 ）mmol/g Pr], higher level of MDA were observed in higher fluoride exposed group [ （3.72 ± 0.59）retool/L, （4.01 ± 0.21 ）mmoL/g Pr], the difference being statistically significant（P 〈 0.05）. Conclusion The results indicated that higher amount of fluoride induced an increased level of oxidation, which might result in the decreased capacity of intelligence of rats with fluorosis.
出处
《中国地方病学杂志》
CAS
CSCD
北大核心
2008年第4期371-373,共3页
Chinese Jouranl of Endemiology
基金
科技部973项目（2006CB708513）
国家自然科学基金（30760224）
贵州省科技厅项目[黔科合外G字（2006400107）,黔省专合字（2006）52,黔科合重大专项（2006）6015]：贵阳医学院科学基金（B200701）
关键词
氟中毒
牙
氧化性应激
丙二醛
学习
记忆
Fluorosis, Dental
Oxidative Stress
Malondialdehyde
Learning
Memory
