摘要
目的探讨维生素C、E（VC、VE）干预对氟中毒大鼠肝、肾和脑组织超微结构变化的影响。方法将120只Wistar大鼠分为9组。对照组饮用纯净水；染氟组饮用高氟水（含氟化钠150mg／L）。染氟同时，VC干预组分别用50、100、150mg·kg^-1·d^-1 3种剂量的VC灌胃；VE干预组分别用25、50、75mg·kg^-1·d^-1 3种剂量的VE灌胃：VC和VE联合干预组以100mg·kg^-1·d^-1 VC和50mg·kg^-1·d^-1 VE同时灌胃。9个月后处死大鼠，用透射电子显微镜观察各组大鼠肝、肾、脑组织超微结构变化。结果大鼠饮用高氟水后，肝、肾、脑组织发生了不同程度的超微结构病理变化。①肝细胞水肿，线粒体基质变淡，在肝细胞内可见脂滴，核基质淡，核仁边集，肝血窦内皮明显肿胀；②肾小管细胞核异染色质较多且边集，细胞间隙和质膜内褶增宽，内质网扩张，集合管上皮细胞内的亮细胞胞质极度松散；③脑胶质细胞肿胀，细胞器很少，细胞核异染色质较多、边集。VC、VE各干预组肝、肾、脑组织超微结构变化轻微或无明显变化，其中VC、VE联合干预组各组织超微结构基本与对照组相同。结论氟中毒可引起大鼠肝、肾、脑组织损害。VC、VE单独及联合干预均对氟中毒引起的组织器官损害有一定的保护作用．联合干预效果强于单独干预。
Objective To observe the effects of vitamin C（VC） and E（VE） on the uhrastructure of liver, kidney and brain tissue of fluorosis rats. Methods One hundred and twenty Wistar rots were chosen as the experimental animals and were divided into 9 groups randomly. The control group were given distilled water and the fluoride exposed group were given distilled water containing sodium fluoride 150 mg/L. The three VC-fluoride exposed groups were given VC orally in a dose of 50,100,150 mg·kg^-1·d^-1, respectively, and the three VE-fluoride exposed groups were given VE of 25,50,75 mg·kg^-1·d^-1, respectively. The VC-VE-fluoride exposed group were given VC of 100 mg·kg^-1·d^-1 and VE of 50 mg·kg^-1·d^-1 at the same time of high fluoride water intake. The rats were sacrificed after 9 months and the uhrastructure changes on liver, kidney and brain tissues of each group were observed under transmission electron microscope （TEM）. Results The uhrastructure of liver, kidney and brain showed pathologic changes in the rats that drank water containing high concentrations of sodium fluoride. （1）Edema of hepatocytes, smeared mitochontria and nuclear matrix, lipid droplet in cytoplasm of hepatocytes, margination of nucleolus as well as obvious swelling of liver sinusoidal endothelial were observed in fluoride exposed group. （2） There were margination of heterochromatin, expansion of cell space and endoplasmic reticulum in the kidney after the exposure to excess fluoride.（3）Significant changes were found on glial cells on the brain, including cell swelling, increase and margination of heterochromatin in the fluoride exposure group. There were no significant uhrastructure changes in the VC or VE intervention group, while the VC-VE-fluoride exposure group was almost the same as the control group. Conclusions Fluorosis may cause damage on liver, kidney and brain in rats. VC and VE, alone or combined, have protective effects, and the combined supplementation was stronger than single supplementations.
出处
《中国地方病学杂志》
CAS
CSCD
北大核心
2008年第4期378-381,共4页
Chinese Jouranl of Endemiology
基金
新疆维吾尔自治区高校科研计划青年启动基金（XJEDU2004S17）
