摘要
目的建立玉屏风汤剂的HPLC指纹图谱分析方法,研究黄芪、防风、白术饮片与全方汤剂指纹图谱的相关性。方法采用HPLC法分析,色谱柱为Hypersil ODS;流动相为乙腈-水(梯度洗脱);检测波长为220nm;流速为1mL·min-1;柱温为30℃。结果药材黄芪、防风、白术(炒)饮片分别标示出9,8,7个主要共有峰;玉屏风汤剂标示出11个共有峰,其中有6个为黄芪特征峰,4个为防风特征峰,1个为白术特征峰。结论黄芪、防风饮片与玉屏风汤剂的HPLC指纹图谱具有一定相关性,玉屏风汤剂的主要特征峰为黄芪异黄酮类及防风色原酮类成分,为玉屏风汤剂配伍规律与物质基础的研究提供了一定参考。
Objective To establish the method of fingerprint analysis on Yupingfeng Decoction, and to study the correlation of HPLC fingerprint in Radix Astragali, Radix Saposhnikovlae, Rhizoma Atractylodis Macrocephalae and Yupingfeng Decoction. Methods HPLC with Hypersil ODS was used, acetonitrile - water（gradient elution） as a mobile phase and detection wavelength at 220 nm, flow rate was 1 mL · min^-1, and column temperature was 30 ℃. Results There were 9, 8 and 7 common peaks separated from 10 batches of medicinal material of Radix Astragali, Radix Saposhnikovlae, and Rhi- zoma Atractylodis Macrocephalae, respectively; 11 common peaks were separated from 10 batches of Yupingfeng Decoction, of which 6 peaks were shared by Radix Astragali, 4 peaks by Radix Saposhnikoviae and 1 peak by Rhizoma Atractylodis Macrocephalae. Conclusion There exists a correlation of Yupingfeng decoction with the medicinal pieces of Radix Astragali and Radix Saposhnikoviae. The major characteristic fingerprint peaks of Yupingfeng decoction belong to those of the isoflavones from Radix Astragali and chromones from Radix Saposhnikoviae. This will provide a reference for the rules of the compatibility and component research of Yupingfeng Decoction.
出处
《中药新药与临床药理》
CAS
CSCD
2008年第4期283-288,共6页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
广东省科技计划项目(2007B060401010)
关键词
玉屏风汤剂
黄芪
防风
白术
指纹图谱
HPLC
Yupingfeng Decoction
Radix Astragali
Radix Saposhnikoviae
Rhizoma Atractylodis Macrocephalae
Fingerprint
HPLC
