摘要
目的了解Wilson病(WD)免疫功能及二巯基丙磺酸钠(DMPS)短期驱铜治疗和长期综合驱铜治疗对其影响;进而评价DMPS短期驱铜治疗及长期综合驱铜治疗的有效性、安全性、科学性。方法选取正常对照组19名、未经治疗和长期正规综合驱铜治疗≥2年的WD患者各21例,长期综合驱铜治疗WD患者、未经治疗WD患者在接受DMPS短期治疗前、后及正常对照组均采取空腹静脉血:散射比浊法测定血清免疫球蛋白、补体水平及流式细胞术进行外周静脉全血T淋巴细胞亚群、CD3-CD16+CD56+及CD19+分析。结果WD未经治疗组体液免疫指标之CD19+、IgG、IgM均较正常对照组显著升高,C4较正常对照组显著减低;细胞免疫指标之CD4+、CD3-CD16+CD56+及CD4+/CD8+均较正常对照组减低。DMPS短期驱铜治疗后CD19+、IgG显著减低。长期综合驱铜治疗后CD19+、IgG、IgM、C3、CD4+、CD4+/CD8+、CD3-CD16+CD56+均有显著恢复。结论WD免疫功能紊乱:表现为体液免疫亢进,细胞免疫低下;DMPS短期驱铜治疗仅可恢复部分免疫学指标;长期综合驱铜治疗对免疫功能有显著恢复。从免疫学角度看,长期综合驱铜治疗是安全、必要的。
Objective To investigate the immune function of patients with Wilson＇ s Disease（WD） ,and the effects of short term decoppering therapy with Sodium dimercaptosulphonate（DMPS） and long term complex decoppering therapy in hopes of evaluating their validity, security and scientificness. Methods 21 WD patients without treatment previously（ group A） ,21 WD patients with 2 years or more of routine complex decoppering therapy history （group B ） and 19 normal individual （control group） were enrolled in this experiment. Group A were given the short term of decoppering therapy with DMPS. The concentration of serum immunoglobulins and complement were measured by immune turbidimetry, peripheral blood T-lymphocyte subsets, CD3^- , CD16^＋ , CD56^＋ , CD19^＋ were determined by flow cytometer. Results CD19^＋ , IgG and IgM in group A were higher than that in control,but C4,CD4^＋ , CD3^- CD16^＋CD56^＋ and the ratio of CD4^＋/CD8^＋ in group A were lower than that in control group. After the short term of decoppering therapy with DMPS, CD19^＋ and IgG in group A decreased. CD4^＋ , CD19^＋ , CD3^- CD16^＋ CD56^＋ , IgG, IgM, C4 and the ratio of CD4^＋/CD8^＋ were all recovered distinctly after the long term of complex decoppering therapy（ group B）. Conclusions The study showed that there is the disorder of the immunological state in WD,manifested by the activation of humoral inlnlunity and depression of cellular immunity. The short term decoppering therapy with DMPS could ameliorate some of the immunological indexes. The immunological homeostasis had recovered obviously by the long - term complex decoppering therapy,which is safe and indispensable for the WD patient.
出处
《中华全科医学》
2008年第8期776-777,共2页
Chinese Journal of General Practice
关键词
WILSON病
免疫功能
流式细胞术
二巯基丙磺酸钠
Wilson＇s Disease（WD）
Immune function
Flow cytometry（FCM）
Sodium dimercaptosulphonate
