摘要
目的:促红细胞生成素(Epo)是促红细胞生成的因子,由胎儿肝脏和成人肾脏产生,是贫血及缺氧时的一种应答反应。视网膜异常血管形成,如早产儿视网膜病变(ROP),增殖性糖尿病性视网膜病变(PDR)时Epo水平增高,提示Epo在病理性眼部血管生成中的作用。Epo参与视网膜血管生成,但其与角膜新生血管是否有关尚未见报道。本研究旨在探讨Epo/EpoR是否在正常和新生血管化角膜表达及角膜内注射Epo是否可诱发角膜新生血管的产生,从而了解其与角膜新生血管的联系。方法:(1)制备碱烧伤诱导鼠角膜新生血管模型,免疫组织化学的方法检测Epo及EpoR是否在正常角膜及新生血管化角膜表达;(2) Epo的克隆、表达及纯化;(3)角膜基质内分别注射Epo(6μL,1μg)及Epo对照(载体对照)及盐水,第14d观察角膜是否有新生血管产生。结果:Epo及EpoR在正常角膜及碱诱发的新生血管化角膜的角膜上皮细胞,角膜内皮细胞,基质细胞均有表达,并在新生血管化角膜表达加强,同时也在基质内炎症细胞及新生血管均有表达。角膜基质内注射Epo后第14d,6眼中5眼产生新生血管,对照组6眼均未见新生血管。结论:本文首次报道了Epo及其受体表达于正常角膜和新生血管化角膜。角膜内注射注射Epo可诱发角膜新生血管。Epo及其受体系统与角膜新生血管化的形成有关。
AIM： To test the expression of erythropoietin （Epo） and its receptor EpoR in normal and neovascularized murine corneas induced by alkali burns, and to investigate whether Epo/EpoR is involved in the process of corneal angiogenesis.
METHODS： The expression of Epo/EpoR was tested in normal and neovascularized murine corneas induced by alkali burns through immunohistochemistry of corneal frozen sections. Epo cloning, expression, and purification were carried out. Then Epo protein （6μL, 1μg） and control （6μ L of vector control or saline） were injected into the corneal stroma respectively, and the corneas were checked at the 14m day after injection to see whether corneal neovascuarization occurred.
RESULTS： Epo/EpoR was expressed in epithelial cells, endothelial cells and stromal cells in normal and neovascularized corneas induced by alkaline burns, and also expressed strongly in neovascularized cornea. They were expressed at the same time in stromal inflammatory cells and new vessels. Corneal neovascularization was induced by Epo intrastromal injection in 5 out of 6 eyes , but no new vessels were observed in all controls （ n = 6） at day 14 after vector control or saline intrastromal injection in normal corneas.
CONCLUSION： This paper first reported the expression of Epo and its receptor in normal and neovascularized cornea. Injection of Epo into the corneal stroma mayinduce the corneal neovascularization, Epo/EpoR is associated with the formation of corneal neovascularization.
出处
《国际眼科杂志》
CAS
2008年第5期857-860,共4页
International Eye Science
