摘要
目的观察血管紧张素-(1-7)[Ang-(1-7)]对血管紧张素-Ⅱ(Ang-Ⅱ)诱导的肾小球系膜细胞(GMC)增殖过程中c-fos表达的影响。方法体外培养GMC,Ang-Ⅱ为刺激因子,不同浓度Ang-(1-7)为干预因子。用结晶紫计数法检测GMC数目的变化;Western blot检测GMC中c-fos蛋白表达的变化。结果Ang-(1-7)呈剂量依赖性地抑制Ang-Ⅱ诱导的GMC增殖和GMC内c-fos蛋白表达。结论Ang-(1-7)可能通过抑制c-fos的表达,抑制Ang-Ⅱ诱导的GMC增殖。
Objective To investigate the effect of angiotensin-(1-7) on the expression of cellular c-fos in angiotensin Ⅱ-induced proliferative glomerular mesangial cells (GMC). Methods GMC were treated with angiotensin Ⅱ and different dose of angiotensin-( 1-7 ).GMC number were evaluated by crystal violet staining and the expression of c-fos were detected by western blot..Results Angiotensin-(1-7)inhibit angiotensin II Ⅱ-induced GMC proliferation as well as the expression c-fos in a concentration dependent manner. Conclusion c-fos is involved in the inhibiting effects of angiotensin-(1-7) on angiotensin Ⅱ-induced GMC proliferation.
出处
《中国医师杂志》
CAS
2008年第7期868-870,共3页
Journal of Chinese Physician