摘要
目的研究强啡肽及P物质在尿毒症皮肤瘙痒中的相互作用。方法5／6肾大部切除模型（STNx），24周进入终末期肾功能衰竭（ESRD）。分为A：STNx＋P，B：STNx＋强，C：sTNx模型对照及D：假手术组。分别皮内注射P物质（SP）、强啡肽及生理盐水。注射后30min内观察搔抓反应。ELISA法检测血中sP浓度，免疫组化法检测皮肤组织SP表达。结果5／6肾切除大鼠血中SP升高，皮内注射SP的大鼠进一步升高，而皮内注射强啡肽后可明显降低血中SP浓度（A-D：2530．0±236．3 vs 612．4±72．2 vs 1010．2±103．5 vs 240．2±36．5）。免疫组化皮肤组织SP的表达可见到相似的趋势。STNx＋P组大鼠搔抓次数最多，B组与C组差异无统计学意义（P〉0．05）。结论尿毒症大鼠皮肤瘙痒与其血中及皮肤组织中SP表达增高有关。皮内注射强啡肽不能诱发尿毒症大鼠皮肤瘙痒反应，并能降低其血中及皮肤组织中SP的水平。
Objective To investigate the role of dynophin and substance P in pruritus with 5/6 subtotal nephrectomy rats （STNx）.Methods 5/6 subtotal nephrectomy rats were prepared. After 24 weeks , STNx rats entered in end stage renal disease（ESRD）. Sixty male SD rats were divided into 4 groups, STNx ＋ P group（ n = 15 ）, STNx ＋ dynophin group（ n =15 ）, STNx control group（ n = 15 ） and sham operation group（ n = 15 ）. Substance P （SP）, dynophin and saline were intradermal injected separately. After injection, scratch reaction of rats in 30 minutes were recorded. And then SP concentration in blood was estimated by ELISA and SP expression in skin was examined by immunohistochemisty. Results SP concentration in blood of STNx rats were （ 1010.2 ±103.5） pg/ml, which increased to （2530.0±236.3） pg/ml in STNx ＋ SP group and decreased to （612.4±72.2） pg/ml in STNx ＋ dynophin group, and it was the lowest in sham operation group （240.2±36.5） pg/ml. SP expression in skin was similar to that of in blood. The scratch times in STNx ＋ SP group were highest （7.3±1.9 times）, there was no significant difference between STNx ＋ dynophin group and STNx control group. Conclusion Pruritus in ESRD rats was correlated to the increase of SP in blood and skin. Intradermal injection of dynophin can decrease SP in blood and skin, but can not induce scratch in STNx rats.
出处
《中国医师杂志》
CAS
2008年第7期895-897,共3页
Journal of Chinese Physician
基金
基金项目：湖北省卫生厅科研基金资助项目（2005-455#）
湖北省武汉市“511”人才基金资助项目（武卫2007-22#）.
